Document Detail


Erianin induces apoptosis in human leukemia HL-60 cells.
MedLine Citation:
PMID:  11749794     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate the effect of erianin on human HL-60 cell line and explore its mechanism of apoptosis in vitro. METHODS: Inhibition of proliferation was measured with colorimetric MTT assay. The morphologic changes were observed by fluorescence and electron microscopes. DNA fragmentation was visualized by agarose gel electrophoresis, and the DNA degradation was determined by flow cytometry. Immunohistochemical analysis was used to identify the expression of bcl-2 and bax genes. RESULTS: The growth of human HL-60 cells was significantly inhibited by erianin 20-81.9 nmol/L during 72 h treatment (P < 0.01). The IC50 value was 38 nmol/L after a 24-h exposure to erianin, while that of vincristine, the positive control, was 101 nmol/L. The typical morphologic changes were observed and the nuclear DNA fragmentation exhibited "ladder" pattern. The cell cycle of HL-60 cells was arrested in G2/M phase, and expression of bcl-2 gene was decreased while that of bax was increased. CONCLUSION: Erianin showed potent inhibitory activity on the proliferation of HL-60 cells. The inhibition might be relative to the apoptosis induced by erianin and the altered expression of bcl-2 and bax genes in HL-60 cells.
Authors:
Y M Li; H Y Wang; G Q Liu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  22     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-12-25     Completed Date:  2003-10-02     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  1018-22     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis*
Bibenzyls / pharmacology*
Cell Division / drug effects
Dendrobium / chemistry*
HL-60 Cells
Humans
Proto-Oncogene Proteins / biosynthesis,  genetics
Proto-Oncogene Proteins c-bcl-2 / biosynthesis,  genetics
bcl-2-Associated X Protein
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/BAX protein, human; 0/Bibenzyls; 0/Erianin; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein

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