Document Detail

Eri1 degrades the stem-loop of oligouridylated histone mRNAs to induce replication-dependent decay.
MedLine Citation:
PMID:  23202588     Owner:  NLM     Status:  MEDLINE    
The exoRNase Eri1 inhibits RNA interference and trims the 5.8S rRNA 3' end. It also binds to the stem-loop of histone mRNAs, but the functional importance of this interaction remains elusive. Histone mRNAs are normally degraded at the end of S phase or after pharmacological inhibition of replication. Both processes are impaired in Eri1-deficient mouse cells, which instead accumulate oligouridylated histone mRNAs. Eri1 trims the mature histone mRNAs by two unpaired nucleotides at the 3' end but stalls close to the double-stranded stem. Upon oligouridylation of the histone mRNA, the Lsm1-7 heteroheptamer recognizes the oligo(U) tail and interacts with Eri1, whose catalytic activity is then able to degrade the stem-loop in a stepwise manner. These data demonstrate how degradation of histone mRNAs is initiated when 3' oligouridylation creates a cis element that enables Eri1 to process the double-stranded stem-loop structure.
Kai P Hoefig; Nicola Rath; Gitta A Heinz; Christine Wolf; Jasmin Dameris; Aloys Schepers; Elisabeth Kremmer; K Mark Ansel; Vigo Heissmeyer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-12-02
Journal Detail:
Title:  Nature structural & molecular biology     Volume:  20     ISSN:  1545-9985     ISO Abbreviation:  Nat. Struct. Mol. Biol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-04     Completed Date:  2013-02-25     Revised Date:  2014-02-14    
Medline Journal Info:
Nlm Unique ID:  101186374     Medline TA:  Nat Struct Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  73-81     Citation Subset:  IM    
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MeSH Terms
Cell Cycle
Cells, Cultured
Exonucleases / genetics,  metabolism*
Histones / genetics*,  metabolism
Inverted Repeat Sequences*
Lymphocyte Activation
Mice, Inbred C57BL
Nucleic Acid Conformation
Oligoribonucleotides / metabolism
RNA Processing, Post-Transcriptional
RNA, Messenger / chemistry*,  genetics,  metabolism*
RNA, Ribosomal / genetics,  metabolism
T-Lymphocytes, Helper-Inducer / immunology,  metabolism
Uracil Nucleotides / metabolism
Grant Support
Reg. No./Substance:
0/Histones; 0/Oligoribonucleotides; 0/RNA, Messenger; 0/RNA, Ribosomal; 0/Uracil Nucleotides; 0/oligo(U); EC 3.1.-/Eri1 protein, mouse; EC 3.1.-/Exonucleases

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