| ErbB signaling has a role in radial sorting independent of Schwann cell number. | |
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MedLine Citation:
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PMID: 21491500 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the peripheral nervous system, Schwann cells make myelin, a specialized sheath that is essential for rapid axonal conduction of action potentials. Immature Schwann cells initially interact with many axons, but, through a process termed radial sorting, eventually interact with one segment of a single axon as promyelinating Schwann cells. Previous studies have identified genes that are required for Schwann cell process extension and proliferation during radial sorting. Previous analyses also show that ErbB signaling is required for Schwann cell proliferation, myelination, radial sorting, and the proper formation of unmyelinated Remak bundles. Because ErbB signaling and Schwann cell proliferation are both required during radial sorting, we sought to determine if the primary function of ErbB signaling in this process is to regulate Schwann cell proliferation or if ErbB signaling also controls other aspects of radial sorting. To address this question, we applied small molecule inhibitors in vivo in zebrafish to independently block ErbB signaling and proliferation. Ultrastructural analysis of treated animals revealed that both ErbB signaling and Schwann cell proliferation are required for radial sorting in vivo. ErbB signaling, however, is required for Schwann cell process extension, while Schwann cell proliferation is not. These results provide in vivo evidence that ErbB signaling plays a direct role in process extension during radial sorting, in addition to its role in regulating Schwann cell proliferation. |
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Authors:
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Alya R Raphael; David A Lyons; William S Talbot |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-04-12 |
Journal Detail:
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Title: Glia Volume: 59 ISSN: 1098-1136 ISO Abbreviation: Glia Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-05-13 Completed Date: 2011-09-06 Revised Date: 2012-09-18 |
Medline Journal Info:
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Nlm Unique ID: 8806785 Medline TA: Glia Country: United States |
Other Details:
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Languages: eng Pagination: 1047-55 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Wiley-Liss, Inc. |
Affiliation:
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Department of Developmental Biology, Stanford University, Stanford, California, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acridine Orange
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diagnostic use Animals Animals, Genetically Modified Axons / drug effects, metabolism Cell Count Cell Movement / physiology Cell Proliferation* / drug effects Early Growth Response Protein 2 / metabolism Embryo, Nonmammalian Enzyme Inhibitors / pharmacology Forkhead Transcription Factors / genetics Gene Expression Regulation, Developmental / drug effects Green Fluorescent Proteins / genetics Lateral Line System / cytology, drug effects, physiology Microscopy, Confocal / methods Microscopy, Electron, Transmission Myelin Basic Proteins / metabolism Myelin Sheath / metabolism Oncogene Proteins v-erbB / metabolism* Organic Cation Transport Proteins / metabolism Schwann Cells / drug effects, metabolism*, ultrastructure Signal Transduction / drug effects, physiology* Statistics, Nonparametric Tubulin / metabolism Zebrafish Zebrafish Proteins / genetics |
| Grant Support | |
ID/Acronym/Agency:
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NS050223/NS/NINDS NIH HHS; R01 NS050223-07/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Early Growth Response Protein 2; 0/Enzyme Inhibitors; 0/Forkhead Transcription Factors; 0/Myelin Basic Proteins; 0/Oncogene Proteins v-erbB; 0/Organic Cation Transport Proteins; 0/Tubulin; 0/Zebrafish Proteins; 0/foxd3 protein, zebrafish; 147336-22-9/Green Fluorescent Proteins; 65-61-2/Acridine Orange |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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