Document Detail


Eradication of Rickettsia tsutsugamushi from patients' blood by chemotherapy, as assessed by the polymerase chain reaction.
MedLine Citation:
PMID:  7741170     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The presence of Rickettsia tsutsugamushi DNA in peripheral blood mononuclear cells of eight patients with tsutsugamushi disease was determined by the polymerase chain reaction during antibiotic treatment with minocycline or doxycycline. Rickettsia tsutsugamushi DNA was detectable in all samples from these patients collected the day before treatment began. After the initiation of chemotherapy, all samples tested positive on the third or fourth day, and one sample tested positive on the eighth day, showing a slow action of the drugs against the rickettsia within cells. Immune responses against R. tsutsugamushi also seemed to be important for eradication of the pathogens, as suggested by patients' high antibody titers.
Authors:
K Murai; A Okayama; H Horinouchi; T Oshikawa; N Tachibana; H Tsubouchi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of tropical medicine and hygiene     Volume:  52     ISSN:  0002-9637     ISO Abbreviation:  Am. J. Trop. Med. Hyg.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-06-06     Completed Date:  1995-06-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370507     Medline TA:  Am J Trop Med Hyg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  325-7     Citation Subset:  AIM; IM    
Affiliation:
Second Department of Internal Medicine, Miyazaki Medical School, Japan.
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Antibodies, Bacterial / blood
Base Sequence
DNA Primers / chemistry
DNA, Bacterial / blood*,  chemistry
Doxycycline / therapeutic use
Electrophoresis, Agar Gel
Female
Fluorescent Antibody Technique
Humans
Leukocytes, Mononuclear / microbiology*
Male
Middle Aged
Minocycline / therapeutic use
Molecular Sequence Data
Orientia tsutsugamushi / genetics,  immunology,  isolation & purification*
Polymerase Chain Reaction*
Scrub Typhus / drug therapy,  microbiology*
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/DNA Primers; 0/DNA, Bacterial; 10118-90-8/Minocycline; 564-25-0/Doxycycline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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