Document Detail

Equine carpal articular cartilage fibronectin distribution associated with training, joint location and cartilage deterioration.
MedLine Citation:
PMID:  10661385     Owner:  NLM     Status:  MEDLINE    
Processes involved in equine carpal osteochondral injury have not been established. In other species, fibronectin appears important in chondrocyte-matrix interactions, and levels are increased in osteoarthritis. This investigation aimed to (a) describe fibronectin immunoreactivity in the middle carpal joint of 2-year-old Thoroughbreds, (b) determine topographical variations, (c) compare strenuously trained (Group 1) or gently exercised horses (Group 2) and (d) describe sites with early osteoarthritis. Group 1 (n = 6) underwent a 19 week high intensity treadmill training programme. Group 2 (n = 6) underwent 40 min walking until euthanasia. Dorsal and palmar sites on radial, intermediate and third carpal articular surfaces were prepared. Immunohistochemistry was performed using a biotin-streptavidin/peroxidase method. Cross-reactivity of rabbit antihuman fibronectin antiserum with equine fibronectin was confirmed using Western blotting. Results showed: (a) fibronectin was present primarily in pericellular and interterritorial matrix locations, (b) dorsal sites had zonal immunoreactivity compared to palmar sites, (c) Group 1 dorsal radial carpal cartilage had increased superficial staining compared to Group 2 and (d) fibrillated cartilage showed increased intracellular and local matrical immunoreactivity (superficial zone). These findings suggest topographical and exercise-related variations in fibronectin distribution, and indicate equine fibronectin is localised at sites of cartilage degeneration and released into the matrix by chondrocytes in the local area.
R C Murray; H C Janicke; F M Henson; A Goodship
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Equine veterinary journal     Volume:  32     ISSN:  0425-1644     ISO Abbreviation:  Equine Vet. J.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-03-02     Completed Date:  2000-03-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0173320     Medline TA:  Equine Vet J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  47-51     Citation Subset:  IM    
Centre for Equine Studies, Animal Health Trust, Kentford, Newmarket, UK.
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MeSH Terms
Blotting, Western / veterinary
Carpus, Animal / chemistry*,  immunology,  pathology
Cartilage, Articular / chemistry*,  immunology,  physiopathology
Fibronectins / analysis*,  immunology
Horse Diseases / immunology,  metabolism*,  physiopathology
Joint Diseases / metabolism,  physiopathology,  veterinary*
Osteoarthritis / veterinary
Physical Conditioning, Animal
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