| Equine carpal articular cartilage fibronectin distribution associated with training, joint location and cartilage deterioration. | |
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MedLine Citation:
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PMID: 10661385 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Processes involved in equine carpal osteochondral injury have not been established. In other species, fibronectin appears important in chondrocyte-matrix interactions, and levels are increased in osteoarthritis. This investigation aimed to (a) describe fibronectin immunoreactivity in the middle carpal joint of 2-year-old Thoroughbreds, (b) determine topographical variations, (c) compare strenuously trained (Group 1) or gently exercised horses (Group 2) and (d) describe sites with early osteoarthritis. Group 1 (n = 6) underwent a 19 week high intensity treadmill training programme. Group 2 (n = 6) underwent 40 min walking until euthanasia. Dorsal and palmar sites on radial, intermediate and third carpal articular surfaces were prepared. Immunohistochemistry was performed using a biotin-streptavidin/peroxidase method. Cross-reactivity of rabbit antihuman fibronectin antiserum with equine fibronectin was confirmed using Western blotting. Results showed: (a) fibronectin was present primarily in pericellular and interterritorial matrix locations, (b) dorsal sites had zonal immunoreactivity compared to palmar sites, (c) Group 1 dorsal radial carpal cartilage had increased superficial staining compared to Group 2 and (d) fibrillated cartilage showed increased intracellular and local matrical immunoreactivity (superficial zone). These findings suggest topographical and exercise-related variations in fibronectin distribution, and indicate equine fibronectin is localised at sites of cartilage degeneration and released into the matrix by chondrocytes in the local area. |
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Authors:
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R C Murray; H C Janicke; F M Henson; A Goodship |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Equine veterinary journal Volume: 32 ISSN: 0425-1644 ISO Abbreviation: Equine Vet. J. Publication Date: 2000 Jan |
Date Detail:
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Created Date: 2000-03-02 Completed Date: 2000-03-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0173320 Medline TA: Equine Vet J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 47-51 Citation Subset: IM |
Affiliation:
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Centre for Equine Studies, Animal Health Trust, Kentford, Newmarket, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western / veterinary Carpus, Animal / chemistry*, immunology, pathology Cartilage, Articular / chemistry*, immunology, physiopathology Female Fibronectins / analysis*, immunology Horse Diseases / immunology, metabolism*, physiopathology Horses Immunohistochemistry Joint Diseases / metabolism, physiopathology, veterinary* Osteoarthritis / veterinary Physical Conditioning, Animal Random Allocation |
| Chemical | |
Reg. No./Substance:
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0/Fibronectins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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