Document Detail


Equine blastocyst development after intracytoplasmic injection of sperm subjected to two freeze-thaw cycles.
MedLine Citation:
PMID:  16095679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was conducted to evaluate the effects of thawing, division into aliquots and refreezing on fertilizing capacity (ability to support embryo development after intracytoplasmic sperm injection; ICSI) of frozen stallion semen. Frozen semen from a fertile stallion was thawed, diluted 1:100 with freezing extender, and refrozen (2F treatment). Control semen was frozen only once. In vitro matured equine oocytes were injected with: (1) motile control spermatozoa; (2) motile 2F spermatozoa; (3) non-motile 2F spermatozoa; or (4) non-motile 2F spermatozoa, followed by injection of sperm extract. Blastocyst development after ICSI was equivalent between control spermatozoa and motile 2F spermatozoa (27 and 23%, respectively). Blastocyst development after injection of non-motile 2F spermatozoa (13%) tended (P=0.07) to be lower than that for control spermatozoa. Injection of sperm extract into oocytes that received non-motile 2F spermatozoa resulted in a significant decrease in blastocyst development (to 2%) compared with injection of non-motile 2F spermatozoa alone. Spermatozoa from a subfertile stallion was similarly processed and used for ICSI; blastocyst development for both motile control (once frozen) spermatozoa and motile 2F spermatozoa was 9%. In conclusion, frozen stallion semen may be thawed, diluted, and refrozen without effect on the ability of motile spermatozoa to initiate embryo development after ICSI. Non-motile spermatozoa from reprocessed semen may also achieve embryo development after ICSI. To our knowledge, this is the first report evaluating the ability of refrozen spermatozoa to produce embryos by ICSI in any species.
Authors:
Y H Choi; C C Love; D D Varner; K Hinrichs
Related Documents :
10967009 - Maternal serum inhibin a concentrations in early pregnancy after ivf and embryo transfe...
24386309 - Soluble leukocyte-associated ig-like receptor-1 in amniotic fluid is of fetal origin an...
21315339 - Vitrified-warmed blastocyst transfer cycles yield higher pregnancy and implantation rat...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-08-10
Journal Detail:
Title:  Theriogenology     Volume:  65     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-07     Completed Date:  2006-05-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  808-19     Citation Subset:  IM    
Affiliation:
Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX 77843-4466, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / physiology*
Cryopreservation / methods,  veterinary*
Embryonic Development*
Fertility
Horses / embryology*
Male
Semen Preservation / methods,  veterinary*
Sperm Injections, Intracytoplasmic / veterinary*
Sperm Motility

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Impaired female sexual behavior of rat offspring exposed to Solanum lycocarpum unripe fruits during ...
Next Document:  Continuous fetal heart rate monitoring during late gestation in cattle by means of Doppler ultrasono...