| Equilibrium modeling of extractive enzymatic hydrolysis of penicillin G with concomitant 6-aminopenicillanic acid crystallization. | |
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MedLine Citation:
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PMID: 11948446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present downstream processing of penicillin G, penicillin G is extracted from the fermentation broth with an organic solvent and purified as a potassium salt via a number of back-extraction and crystallization steps. After purification, penicillin G is hydrolyzed to 6-aminopenicillanic acid, a precursor for many semisynthetic beta-lactam antibiotics. We are studying a reduction in the number of pH shifts involved and hence a large reduction in the waste salt production. To this end, the organic penicillin G extract is directly to be added to an aqueous immobilized enzyme suspension reactor and hydrolyzed by extractive catalysis. We found that this conversion can exceed 90% because crystallization of 6-aminopenicillanic acid shifts the equilibrium to the product side. A model was developed for predicting the equilibrium conversion in batch systems containing both a water and a butyl acetate phase, with either potassium or D-p-hydroxyphenylglycine methyl ester as counter-ion of penicillin G. The model incorporates the partitioning equilibrium of the reactants, the enzymatic reaction equilibrium, and the crystallization equilibrium of 6-aminopenicillanic acid. The model predicted the equilibrium conversion of Pen G quite reasonably for different values of pH, initial penicillin G concentration and phase volume ratio. The model can be used as a tool for optimizing the enzymatic hydrolysis. |
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Authors:
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M B Diender; A J J Straathof; T van der Does; C Ras; J J Heijnen |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 78 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-04-11 Completed Date: 2002-09-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 395-402 Citation Subset: IM |
Copyright Information:
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Copyright 2002 Wiley Periodicals, Inc. |
Affiliation:
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Delft University of Technology, Kluyver Laboratory for Biotechnology, Julianalaan 67, NL-2628 BC Delft, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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metabolism* Catalysis Chromatography, High Pressure Liquid / methods Computer Simulation Crystallization Escherichia coli / enzymology Fermentation Glycine / analogs & derivatives*, chemistry Hydrogen-Ion Concentration Hydrolysis Models, Chemical* Penicillanic Acid / analogs & derivatives, chemistry, isolation & purification*, metabolism* Penicillin Amidase / metabolism Penicillin G / isolation & purification, metabolism* Potassium / chemistry Reproducibility of Results Sensitivity and Specificity Water / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 123-86-4/butyl acetate; 551-16-6/aminopenicillanic acid; 56-40-6/Glycine; 61-33-6/Penicillin G; 7440-09-7/Potassium; 7732-18-5/Water; 87-53-6/Penicillanic Acid; 938-97-6/4-hydroxyphenylglycine; EC 3.5.1.11/Penicillin Amidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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