| Equilibrium folding of porcine insulin precursor in the presence of redox buffer: implications for the common intermediates shared by its unfolding/ refolding processes. | |
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MedLine Citation:
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PMID: 18991774 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We use the procedure established for 'disulfide stability analysis in redox system' to investigate the unfolding process of porcine insulin precursor (PIP). Six major unfolding intermediates have been captured, in which four contain two disulfides, two contain one disulfide. Based on the characterization and analysis of the intermediates an unfolding pathway has been proposed, by which the native PIP unfolded through in turn 2SS and 1SS intermediates into fully reduced form. Besides, the comparison of the intermediates captured in PIP unfolding process with those intermediates captured in its refolding process revealed that some intermediates captured during both unfolding/refolding processes of PIP have identical disulfide pairing pattern, from which we suggest that the unfolding/refolding processes of PIP share some common intermediates but flow in the opposite direction. |
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Authors:
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Jie Zhao; Qi-Long Huang; Yue-Hua Tang; Zhan-Yun Guo; Zhi-Song Qiao; Gen-Jun Xu; You-Min Feng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Protein and peptide letters Volume: 15 ISSN: 0929-8665 ISO Abbreviation: Protein Pept. Lett. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-11-10 Completed Date: 2008-12-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9441434 Medline TA: Protein Pept Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 972-9 Citation Subset: IM |
Affiliation:
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College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Buffers Chromatography, High Pressure Liquid Glutathione Glutathione Disulfide Mass Spectrometry Oxidation-Reduction Proinsulin / chemistry*, isolation & purification Protein Conformation Protein Denaturation Protein Folding* Recombinant Proteins / chemistry, isolation & purification Swine |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Recombinant Proteins; 27025-41-8/Glutathione Disulfide; 70-18-8/Glutathione; 9035-68-1/Proinsulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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