| Equilibrium clusters in concentrated lysozyme protein solutions. | |
| | |
MedLine Citation:
|
PMID: 21880324 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
We have studied the structure of salt-free lysozyme at 293K and pH 7.8 using molecular simulations and experimental SAXS effective potentials between proteins at three volume fractions, ϕ=0.012, 0.033, and 0.12. We found that the structure of lysozyme near physiological conditions strongly depends on the volume fraction of proteins. The studied lysozyme solutions are dominated by monomers only for ϕ⩽0.012; for the strong dilution 70% of proteins are in a form of monomers. For ϕ=0.033 only 20% of proteins do not belong to a cluster. The clusters are mainly elongated. For ϕ=0.12 almost no individual particles exits, and branched, irregular clusters of large extent appear. Our simulation study provides new insight into the formation of equilibrium clusters in charged protein solutions near physiological conditions. |
| | |
Authors:
|
P Kowalczyk; A Ciach; P A Gauden; A P Terzyk |
Related Documents
:
|
21412434 - Tdp-43 regulates drosophila neuromuscular junctions growth by modulating futsch/map1b l... 22880084 - The "cpc clip motif": a conserved structural signature for heparin-binding proteins. 8392324 - Longitudinal study of relations between human salivary antimicrobial proteins and measu... 21925974 - A new strategy for the on-column exopeptidase cleavage of poly-histidine tagged proteins. 22576084 - Identification of o-linked β-d-n-acetylglucosamine-modified proteins from arabidopsis. 20516594 - Crystallization and preliminary crystallographic studies of the butyrolactone autoregul... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-7-22 |
Journal Detail:
|
Title: Journal of colloid and interface science Volume: - ISSN: 1095-7103 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
|
Created Date: 2011-9-1 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0043125 Medline TA: J Colloid Interface Sci Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
|
Nanochemistry Research Institute, Department of Chemistry, Curtin University of Technology, P.O. Box U1987, Perth, 6845 Western Australia, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Study of the bioeffects of CdTe quantum dots on Escherichia coli cells.
Next Document: Rheological behaviour and spectroscopic investigations of cerium-modified AlO(OH) colloidal suspensi...