Document Detail


Equilibrium of 5,6-hydration of NADH and mechanism of ATP-dependent dehydration.
MedLine Citation:
PMID:  3061454     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
At equilibrium, water addition to the 5,6 double bond of NADH was observed to favor the hydrate by a factor of approximately 100. Hydration generates two epimers of NADHX (beta-6-hydroxy-1,4,5,6-tetrahydronicotinamide adenine dinucleotide). Only the 6S epimer of the hydrate was found to serve as a true substrate for an ATP-dependent dehydratase from yeast that regenerates NADH. Yet enzymatic conversion of both epimers of the hydrate to NADH was found to proceed essentially to completion in the presence of ATP and dehydratase. This is explained by the observed ability of the epimers to undergo rapid spontaneous equilibration, so that it is unnecessary to postulate a lack of stereospecificity in the dehydratase.
Authors:
S A Acheson; H N Kirkman; R Wolfenden
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  27     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1989-02-21     Completed Date:  1989-02-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7371-5     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of North Carolina School of Medicine, Chapel Hill 27514.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate Ribose / analogs & derivatives,  metabolism
Adenosine Triphosphate / pharmacology*
Chromatography, High Pressure Liquid
Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
Hydro-Lyases / metabolism
Hydrogen-Ion Concentration
Kinetics
NAD / analogs & derivatives*,  metabolism
Saccharomyces cerevisiae / enzymology
Stereoisomerism
Substrate Specificity
Water / metabolism*
Grant Support
ID/Acronym/Agency:
GM11858/GM/NIGMS NIH HHS; GM18325/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
20762-30-5/Adenosine Diphosphate Ribose; 53-84-9/NAD; 54298-36-1/6-hydroxy-1,4,5,6-tetrahydronicotinamide adenine dinucleotide; 56-65-5/Adenosine Triphosphate; 73435-45-7/NADX; 7732-18-5/Water; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases; EC 4.2.1.-/Hydro-Lyases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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