| Epstein-Barr virus nuclear antigen 2 transactivates latent membrane protein LMP1. | |
| | |
MedLine Citation:
|
PMID: 2352328 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Several lines of evidence are compatible with the hypothesis that Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA-2) or leader protein (EBNA-LP) affects expression of the EBV latent infection membrane protein LMP1. We now demonstrate the following. (i) Acute transfection and expression of EBNA-2 under control of simian virus 40 or Moloney murine leukemia virus promoters resulted in increased LMP1 expression in P3HR-1-infected Burkitt's lymphoma cells and the P3HR-1 or Daudi cell line. (ii) Transfection and expression of EBNA-LP alone had no effect on LMP1 expression and did not act synergistically with EBNA-2 to affect LMP1 expression. (iii) LMP1 expression in Daudi and P3HR-1-infected cells was controlled at the mRNA level, and EBNA-2 expression in Daudi cells increased LMP1 mRNA. (iv) No other EBV genes were required for EBNA-2 transactivation of LMP1 since cotransfection of recombinant EBNA-2 expression vectors and genomic LMP1 DNA fragments enhanced LMP1 expression in the EBV-negative B-lymphoma cell lines BJAB, Louckes, and BL30. (v) An EBNA-2-responsive element was found within the -512 to +40 LMP1 DNA since this DNA linked to a chloramphenicol acetyltransferase reporter gene was transactivated by cotransfection with an EBNA-2 expression vector. (vi) The EBV type 2 EBNA-2 transactivated LMP1 as well as the EBV type 1 EBNA-2. (vii) Two deletions within the EBNA-2 gene which rendered EBV transformation incompetent did not transactivate LMP1, whereas a transformation-competent EBNA-2 deletion mutant did transactivate LMP1. LMP1 is a potent effector of B-lymphocyte activation and can act synergistically with EBNA-2 to induce cellular CD23 gene expression. Thus, EBNA-2 transactivation of LMP1 amplifies the biological impact of EBNA-2 and underscores its central role in EBV-induced growth transformation. |
| | |
Authors:
|
F Wang; S F Tsang; M G Kurilla; J I Cohen; E Kieff |
Related Documents
:
|
15578978 - The hiv-1 tat protein: a multifaceted target for novel therapeutic opportunities. 2840098 - Transcription and replication of human immunodeficiency virus-1 in b lymphocytes in vitro. 23564488 - Lack of serotonin transporter alters bdnf expression in the rat brain during early post... 8032248 - Role of subgenomic minus-strand rna in coronavirus replication. 11034548 - Cloning and promoter analysis of the chicken interferon regulatory factor-3 gene. 19859558 - Combination ppargamma and rxr agonist treatment in melanoma cells: functional importanc... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of virology Volume: 64 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1990 Jul |
Date Detail:
|
Created Date: 1990-07-13 Completed Date: 1990-07-13 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 3407-16 Citation Subset: IM; X |
Affiliation:
|
Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antigens, Viral
/
genetics*,
physiology* B-Lymphocytes / physiology* Blotting, Western Cell Division Cell Transformation, Viral Cloning, Molecular Epstein-Barr Virus Nuclear Antigens Gene Expression Regulation, Viral* Genes, Viral Humans Regulatory Sequences, Nucleic Acid Trans-Activators / physiology* Viral Matrix Proteins* Viral Structural Proteins / genetics |
| Grant Support | |
ID/Acronym/Agency:
|
CA01395/CA/NCI NIH HHS; CA01417/CA/NCI NIH HHS; CA17006/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, Viral; 0/EBV-associated membrane antigen, Epstein-Barr virus; 0/Epstein-Barr Virus Nuclear Antigens; 0/Trans-Activators; 0/Viral Matrix Proteins; 0/Viral Structural Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Characterization of envelope proteins of alcelaphine herpesvirus 1.
Next Document: Resolution of poxvirus telomeres: processing of vaccinia virus concatemer junctions by conservative ...