| Epstein-Barr virus in synergy with tumor-promoter-induced malignant transformation of immortalized human epithelial cells. | |
| | |
MedLine Citation:
|
PMID: 9292707 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
It is difficult to study how Epstein-Barr virus (EBV) causes transformation of human epithelial cells. The major difficulty is that cultured human epithelial cells do not express EBV receptor (complement receptor 2, CR2), hence EBV cannot infect such epithelial cells directly. In order to investigate the role of EBV in the transformation of human epithelial cells, pSG-CR2-Hyg carrier was transfected into immortalized human epithelial cells (293 cells) to express EBV receptor. EBV could infect these CR2-positive cells directly, and expressed EBV antigens. EBV-infected epithelial cells grew in piles with multiple cellular layers and lost contact inhibition in vitro. In soft-agar culture containing 12-0-tetradecanoylphorbol 13-acetate (TPA), EBV-infected 293 cells formed more and larger colonies. When EBV-infected 293 cells were transplanted subcutaneously into nude mice, and treated with TPA, poorly differentiated carcinoma was induced. These results suggest that EBV could induce the malignant transformation of immortalized human epithelial cells in synergy with TPA. |
| | |
Authors:
|
B M Li; Z W Ji; Z S Liu; Y Zeng |
Related Documents
:
|
10417257 - Global protein synthesis shutdown in autographa californica nucleopolyhedrovirus-infect... 8215297 - Inhibition of hiv-1 replication in cultured cells with phosphorylated dideoxyuridine de... 10074127 - A single amino acid in the adenovirus type 37 fiber confers binding to human conjunctiv... 17432037 - Pathogenesis of a thai strain of white spot syndrome virus (wssv) in juvenile, specific... 2912767 - Lipid characterization and 14c-acetate metabolism in catfish taste epithelium. 16603667 - The significance of c16 fatty acids in the sn-2 positions of glycerolipids in the photo... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of cancer research and clinical oncology Volume: 123 ISSN: 0171-5216 ISO Abbreviation: J. Cancer Res. Clin. Oncol. Publication Date: 1997 |
Date Detail:
|
Created Date: 1997-09-30 Completed Date: 1997-09-30 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7902060 Medline TA: J Cancer Res Clin Oncol Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 441-6 Citation Subset: IM |
Affiliation:
|
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, P. R. China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Carcinogens / toxicity* Cell Division / drug effects, physiology Cell Transformation, Neoplastic* Cell Transformation, Viral* Cells, Cultured Cocarcinogenesis* DNA, Viral / analysis Epithelium / drug effects, ultrastructure, virology Female Herpesvirus 4, Human* / genetics, metabolism Humans Kidney / drug effects, ultrastructure, virology Male Mice Mice, Inbred BALB C Mice, Nude Receptors, Complement 3d Receptors, Virus / biosynthesis, genetics Tetradecanoylphorbol Acetate / toxicity* |
| Chemical | |
Reg. No./Substance:
|
0/Carcinogens; 0/DNA, Viral; 0/Receptors, Complement 3d; 0/Receptors, Virus; 16561-29-8/Tetradecanoylphorbol Acetate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Expression of the integrin alpha5 subunit and its mediated cell adhesion in hepatocellular carcinoma...
Next Document: Characterization of colorectal-cancer-related cDNA clones obtained by subtractive hybridization scre...