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Epstein-Barr virus, human papillomavirus, and flow cytometric cell cycle kinetics in nasopharyngeal carcinoma and inverted papilloma among Egyptian patients.
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MedLine Citation:
PMID:  9868598     Owner:  NLM     Status:  MEDLINE    
It is widely accepted that the Epstein-Barr virus is etiologically associated with the development of nasopharyngeal carcinoma. The human papillomavirus is also associated with inverted papilloma. We used the polymerase chain reaction technique to detect both viruses in both types of tumors. Flow cytometry was also used to study the DNA pattern and proliferative behavior of the tumors in relation to the viruses. EBV was detected in 13/20 (65%) of NPC specimens, and in none of IP (n = 10) or control specimens (n = 10). This indicates the contribution of EBV as an etiologic factor in NPC. Five cases of NPC (25%) were positive for HPV 16, two of them were EBV positive. Four HPV 16 positive cases were found among cases with inverted papilloma, but none among the control cases. Flow cytometry revealed that all NPC, IP, and control samples were diploid except one aneuploid NPC sample. Proliferative capacity (PC) of primary tumors was predictive of tumor recurrence in NPC. Using 13.6% as a cut-off point for PC, we were able to discriminate between high risk and low risk groups with 100% sensitivity and 86% specificity. PC can be used as a baseline prognostic parameter in NPC, making it possible to modify courses of treatment in an attempt to inhibit tumor recurrence.
S K Kassim; S A Ibrahim; S Eissa; S S Zaki; M A El-Begermy; M H Abdou; M I Hassan; A Khalifa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Disease markers     Volume:  14     ISSN:  0278-0240     ISO Abbreviation:  Dis. Markers     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1999-03-05     Completed Date:  1999-03-05     Revised Date:  2013-12-16    
Medline Journal Info:
Nlm Unique ID:  8604127     Medline TA:  Dis Markers     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  113-20     Citation Subset:  IM    
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MeSH Terms
Cell Cycle*
DNA, Neoplasm / analysis
DNA, Viral / analysis
Flow Cytometry*
Herpesvirus 4, Human / genetics,  isolation & purification*
Middle Aged
Nasopharyngeal Neoplasms / pathology,  virology*
Papilloma, Inverted / pathology,  virology*
Papillomaviridae / genetics,  isolation & purification*
Polymerase Chain Reaction
Reg. No./Substance:
0/DNA, Neoplasm; 0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Dis Markers
Journal ID (iso-abbrev): Dis. Markers
Journal ID (publisher-id): DM
ISSN: 0278-0240
ISSN: 1875-8630
Publisher: IOS Press
Article Information
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Copyright © 1998 Hindawi Publishing Corporation.
Received Day: 9 Month: 12 Year: 1999
Accepted Day: 9 Month: 12 Year: 1999
Print publication date: Year: 1998
Electronic publication date: Day: 7 Month: 6 Year: 2002
Volume: 14 Issue: 2
First Page: 113 Last Page: 120
PubMed Id: 9868598
ID: 3850611
Article Id: 260392
DOI: 10.1155/1998/260392

Epstein-Barr Virus, Human Papillomavirus, and Flow Cytometric Cell Cycle Kinetics in Nasopharyngeal Carcinoma and Inverted Papilloma among Egyptian Patients
S. K. Kassim1
S. A. Ibrahim2
S. Eissa1
S. S. A. Zaki3
M. A. El-Begermy2
M. H. Abdou2
M. I. Hassan1
A. Khalifa
1Oncology Diagnostic Unit (ODU)Department of BiochemistryFaculty of MedicineAin Shams UniversityCairoEgypt
2Otolaryngology DepartmentFaculty of MedicineAin Shams UniversityCairoEgypt
3Pathology DepartmentFaculty of MedicineAin Shams UniversityCairoEgypt

Article Categories:
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Keywords: oncogenic viruses, nasopharyngeal tumors, PCR, PC, flow cytometry.

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