Document Detail

Epstein-Barr virus and gastric carcinoma.
MedLine Citation:
PMID:  11091849     Owner:  NLM     Status:  MEDLINE    
The Epstein-Barr virus (EBV) is detected in the tissue of about 10% of gastric carcinoma cases throughout the world. In each case, 100% of carcinoma cells are infected with EBV. Analysis of EBV in carcinoma biopsies indicates that carcinoma is formed by the proliferation of a single EBV infected cell. These findings suggest that EBV plays an important role in the development of EBV positive gastric carcinomas. The EBV genes expressed are EBV determined nuclear antigen 1 (EBNA1), two small non-polyadenylated RNAs known as EBER1 and EBER2, and the transcripts from the BamHI-A region (BARF0); in addition, some cases also express a small amount of latent membrane protein 2A (LMP2A). Epithelial cells are refractory to EBV infection in vitro. This has hampered the study of the role of EBV in epithelial malignancies. The use of recombinant EBV carrying a selectable marker has enabled this difficulty to be overcome. EBV infected cell clones can be obtained from most carcinoma cell lines examined, and it was found that cell to cell contact was an efficient mode of EBV infection. Furthermore, it was possible to immortalize primary gastric epithelial cells by EBV infection. The cells expressed identical EBV genes to those typically seen in EBV positive gastric carcinoma, and showed accelerated malignant properties, including growth in soft agarose and tumorigenicity in severe combined immunodeficient (SCID) mice. These results suggest that EBV contributes to the maintenance of the malignant phenotype of EBV positive gastric carcinoma.
K Takada
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Molecular pathology : MP     Volume:  53     ISSN:  1366-8714     ISO Abbreviation:  MP, Mol. Pathol.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-12-01     Completed Date:  2000-12-01     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  9706282     Medline TA:  Mol Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  255-61     Citation Subset:  IM    
Department of Tumor Virology, Hokkaido University, Sapporo, Japan.
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MeSH Terms
Biological Markers
Cell Division / physiology
Cell Transformation, Viral / physiology
Epithelial Cells / virology
Gene Expression
Genes, Viral
Herpesviridae Infections / complications*
Herpesvirus 4, Human / genetics,  isolation & purification*
Membrane Proteins / physiology
Mice, SCID
Oncogenic Viruses / genetics
RNA, Viral
Stomach Neoplasms / virology*
Tumor Cells, Cultured
Tumor Virus Infections / complications*
Reg. No./Substance:
0/Biological Markers; 0/Membrane Proteins; 0/RNA, Viral

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