Document Detail

Epstein-Barr virus detection in kidney biopsy specimens correlates with glomerular mesangial injury.
MedLine Citation:
PMID:  9820448     Owner:  NLM     Status:  MEDLINE    
To determine the relationship between the detection of Epstein-Barr virus (EBV)-specific DNA and glomerular injury, 33 renal needle-biopsy specimens that had been formalin-fixed and paraffin-embedded were analyzed using polymerase chain reaction (PCR) with subsequent nonradioactive Southern blot technique. Light microscopic examination and immunofluorescence were also performed. In 30 of 33 renal biopsy specimens, the beta globin gene could be successfully amplified as integrity controls. These 30 patients consisted of 12 patients with immunoglobulin A nephropathy (IgAN), 10 patients with minor glomerular abnormalities, 6 patients with membranous nephropathy, and 2 patients with focal/segmental lesions. EBV was detected in 7 of 12 patients with IgAN (58%), 3 of 6 patients with membranous nephropathy (50%), 0 of 10 patients with minor glomerular abnormalities (0%), and 2 of 2 patients with focal/segmental lesions. EBV detection was not disease specific. The EBV detection ratio of the group with glomerular mesangial lesions (64%; 9 of 14 patients) was significantly greater than those without (19%; 3 of 16 patients; P < 0.012, chi-square test). The EBV detection ratio of the group with glomerular lesions (60%; 12 of 20 patients) was significantly greater than those without (0%; 0 of 10 patients; P < 0.0016, Fisher's exact test), and the EBV detection ratio of the group with fibrinogen deposits observed in immunofluorescence (73%; 11 of 15 patients) was significantly greater than those without (7%; 1 of 15 patients; P < 0.0002, chi-square test). The EBV detection ratio of the group with immunoglobulin deposits (57%; 12 of 21 patients) was also significantly greater than those without (0%; 0 of 9 patients; P < 0.0040, Fisher's exact test). These data suggest that EBV can damage the glomerular mesangium beyond disease units and be mediated by immunoglobulin in patients with various chronic glomerulonephritides.
H Iwama; S Horikoshi; I Shirato; Y Tomino
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of kidney diseases : the official journal of the National Kidney Foundation     Volume:  32     ISSN:  1523-6838     ISO Abbreviation:  Am. J. Kidney Dis.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1998-12-03     Completed Date:  1998-12-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8110075     Medline TA:  Am J Kidney Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  785-93     Citation Subset:  IM    
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
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MeSH Terms
Biopsy, Needle
Blotting, Southern
Chi-Square Distribution
Complement C3 / analysis
DNA, Viral / analysis,  genetics
Epstein-Barr Virus Infections / diagnosis*,  pathology
Fibrinogen / analysis
Fluorescent Antibody Technique
Globins / genetics
Glomerulonephritis / pathology,  virology*
Glomerulonephritis, IGA / pathology,  virology
Glomerulonephritis, Membranoproliferative / pathology,  virology
Glomerulonephritis, Membranous / pathology,  virology
Glomerulosclerosis, Focal Segmental / pathology,  virology
Herpesvirus 4, Human / genetics,  isolation & purification*
Immunoglobulin A / analysis
Immunoglobulin G / analysis
Immunoglobulin M / analysis
Kidney Glomerulus / pathology,  virology*
Middle Aged
Polymerase Chain Reaction
Reg. No./Substance:
0/Complement C3; 0/DNA, Viral; 0/Immunoglobulin A; 0/Immunoglobulin G; 0/Immunoglobulin M; 9001-32-5/Fibrinogen; 9004-22-2/Globins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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