Document Detail


The Epstein-Barr virus (EBV) ORI1yt enhancer is not B-cell specific and does not respond synergistically to the EBV transcription factors R and Z.
MedLine Citation:
PMID:  2159545     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Epstein-Barr virus DR promoter is located upstream of the PstI repeats, and in addition to the TATA box, it contains an upstream region (positions -69 to -220) responsive to EB1 (Z) (the BZLF1-encoded transcription factor) and an enhancer with two functionally distinct domains, A and B. Domain B has been described as a B-cell-specific EB1-responsive element (P. M. Lieberman, J. M. Hardwick, and S. D. Hayward, J. Virol. 63:3040-3050, 1989) activated synergistically by EB1 and R, an EBV early product encoded by the open reading frame BRLF1 (M. A. Cox, J. Leahy, and J. M. Hardwick, J. Virol. 64:313-321, 1990). We show here that domain B is an R-responsive element in HeLa cells and is therefore not an EB1-responsive B-cell-specific element. However, there is an EB1-binding site (ZRE-B) located within the R-responsive enhancer region. ZRE-B can be deleted without affecting the R-dependent enhancer activity. Moreover, there is no cooperation or synergy between R and EB1 when activating the B domain (ZRE-B plus the R-responsive element) positioned as an enhancer. ZRE-B is therefore not part of the R-inducible enhancer. We have tested several subregions of the DR enhancer B domain, either alone or in combination, for their capacity to transmit the R-activating signal to the rabbit beta-globin promoter. We found that the R-responsive element is composed of four protoenhancers that span the whole B domain. These protoenhancers alone are weakly or not responsive to R. One of the protoenhancers contains the overlapping palindromes 5'-TTGTCCcgtGGACAAaTGTCC-3'. However, one palindrome, either alone or duplicated, or the overlapping palindromes did not respond to R.
Authors:
H Gruffat; N Moreno; A Sergeant
Related Documents :
9238065 - A calcium responsive element that regulates expression of two calcium binding proteins ...
24129975 - Microrna signatures in hereditary breast cancer.
24179385 - Egfr and htert expression as a diagnostic approach for non-small cell lung cancer in hi...
16125365 - Characterization of stat5b phosphorylation correlating with expression of cytokine-indu...
21566205 - Tmj development and growth require primary cilia function.
9506755 - Single blastomeres within human preimplantation embryos express different amounts of me...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  64     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-06-14     Completed Date:  1990-06-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2810-8     Citation Subset:  IM    
Affiliation:
Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
B-Lymphocytes / immunology*
Base Sequence
Cloning, Molecular
DNA Probes
DNA, Viral / genetics
Enhancer Elements, Genetic*
Escherichia coli / genetics
Globins / genetics
Herpesvirus 4, Human / genetics*
Humans
Molecular Sequence Data
Mutation
Promoter Regions, Genetic
Restriction Mapping
Templates, Genetic
Transcription Factors / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/DNA Probes; 0/DNA, Viral; 0/Transcription Factors; 9004-22-2/Globins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Induction of protective immunity in animals vaccinated with recombinant vaccinia viruses that expres...
Next Document:  Mutational analysis of cis elements involved in E2 modulation of human papillomavirus type 16 P97 an...