| Epoxide hydrolase: a marker for experimental hepatocarcinogenesis. | |
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MedLine Citation:
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PMID: 6696387 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat liver chemical hepatocarcinogenesis, induced by interrupted feeding of 2-acetylaminofluorene, results in various cellular preneoplastic stages and finally in a hepatoma in about 70 to 90 percent of the rats. The putative precursors of hepatomas, called hyperplastic nodules, appear after 12 weeks of feeding and, after 16 weeks of feeding carcinogen, most of them are persistent. Epoxide hydrolase is a tightly bound endoplasmic reticulum enzyme which is strongly induced in hyperplastic nodules and hepatomas. This enzyme has been purified, high-titre rabbit antiserum prepared to it, and this antiserum used to search for epoxide hydrolase immunodeterminants in the sera from chemically induced nodule or hepatoma bearing rats. An enzyme linked immunosorbent assay (ELISA) assay using this antiserum showed significant titres of circulating microsomal epoxide hydrolase antigen, range 0.01 to 2.50 (mean 1.18 +/- 0.30) micrograms per ml, in all 24 hepatoma bearing rats tested. Eight sera from animals with large hyperplastic nodules were also significantly positive for this antigen, while sera from six normal controls were negative (less than 0.004 microgram per ml serum). A passive hemagglutination inhibition assay with sheep or normal rat red blood cells sensitized with pure rat microsomal epoxide hydrolase was capable of detecting 0.2 microgram hydrolase per ml serum. With this assay, sera from four rats with hepatomas were found to contain 0.8 to 1.6 micrograms epoxide hydrolase immunodeterminants per ml. Control rat sera had no detectable immunodeterminants. Thus epoxide hydrolase, a marker induced during experimental chemical hepatocarcinogenesis and called the preneoplastic antigen, has been shown to be circulating in the tumor bearing host. |
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Authors:
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M J Griffin; N Gengozian |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Annals of clinical and laboratory science Volume: 14 ISSN: 0091-7370 ISO Abbreviation: Ann. Clin. Lab. Sci. Publication Date: 1984 Jan-Feb |
Date Detail:
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Created Date: 1984-02-28 Completed Date: 1984-02-28 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0410247 Medline TA: Ann Clin Lab Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 27-31 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2-Acetylaminofluorene Animals Antibody Specificity Endoplasmic Reticulum / enzymology Enzyme Induction Enzyme-Linked Immunosorbent Assay Epoxide Hydrolases / immunology, isolation & purification* Liver Neoplasms, Experimental / blood, chemically induced, enzymology* Male Microsomes / enzymology Precancerous Conditions / enzymology* Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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AI 19495/AI/NIAID NIH HHS; CA 24459/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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53-96-3/2-Acetylaminofluorene; EC 3.3.2.-/Epoxide Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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