Document Detail


Epitopes immunologically related to glycophorin A on human malignant and non-malignant cells in culture.
MedLine Citation:
PMID:  6210254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nine monoclonal antibodies specific to different antigenic determinants on the cyanogen bromide fragments CNBr3 (1-8) and CNBr1 (9-81) of glycophorin A, the major sialoglycoprotein of human red blood cells, were used for the detection of similar or cross-reactive epitope(s) on nucleated somatic cells. A panel of human malignant and non-malignant cells consisting of erythroleukemia, melanoma and carcinomas of breast, cervix, larynx, nasopharynx and colon, as well as human fibroblasts and mammary cells, were tested using a sensitive enzyme-linked immunoassay. The presence of glycophorin-like epitopes were demonstrated, in varying concentrations, on all cells tested. These epitropes were found to be localized on the cell membranes by indirect immunofluorescence.
Authors:
A L Barsoum; M S Czuczman; V P Bhavanandan; E A Davidson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  34     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1985-02-11     Completed Date:  1985-02-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  789-95     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology
Cells, Cultured
Epitopes / analysis*
Female
Fluorescent Antibody Technique
Glycophorin / analysis*,  immunology
Humans
Immunoenzyme Techniques
Neoplasms / immunology*
Radioimmunoassay
Sialoglycoproteins / analysis*
Grant Support
ID/Acronym/Agency:
CA 15483/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Epitopes; 0/Glycophorin; 0/Sialoglycoproteins

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