| Epitope mapping of human aromatic L-amino acid decarboxylase. | |
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MedLine Citation:
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PMID: 17194446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Autoimmune polyendocrine syndrome type I (APS I) is a rare hereditary condition considered a model disease for organ specific autoimmunity. A wide range of autoantibodies targeting antigens present in the affected organs have been identified. Autoantibodies against aromatic L-amino acid decarboxylase (AADC) are present in about 50% of APS I patients. In order to increase our understanding of autoantibody specificity in APS I, the aim of the present study was to localize target regions on AADC recognized by sera from APS I patients. Using several complementing strategies, we have shown that autoantibodies against AADC mainly recognize conformational epitopes. The major antigenic determinants were detected N-terminally to amino acid residue 237. Replacement of amino acids 227-230 (ERDK) with alanine residues reduced the reactivity towards AADC by >80% in all patient sera tested, suggesting that amino acids 227-230 are an important part of an immunodominant epitope. |
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Authors:
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Eirik Bratland; Anette S Bøe Wolff; Jan Haavik; Olle Kämpe; Filip Sköldberg; Jaakko Perheentupa; Geir Bredholt; Per M Knappskog; Eystein S Husebye |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-12-22 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 353 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-01-12 Completed Date: 2007-03-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 692-8 Citation Subset: IM |
Affiliation:
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Department of Medicine, Haukeland University Hospital, N-5021 Bergen, Norway. eirik.bratland@student.uib.no |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aromatic-L-Amino-Acid Decarboxylases
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genetics,
immunology* Autoantibodies Carboxy-Lyases / genetics, immunology Epitope Mapping / methods* Humans Models, Molecular Mutagenesis, Site-Directed Polyendocrinopathies, Autoimmune / enzymology, immunology Recombinant Fusion Proteins / immunology |
| Chemical | |
Reg. No./Substance:
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0/Autoantibodies; 0/Recombinant Fusion Proteins; EC 4.1.1.-/Carboxy-Lyases; EC 4.1.1.28/Aromatic-L-Amino-Acid Decarboxylases; EC 4.1.1.29/sulfoalanine decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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