Document Detail

Epitope mapping of human aromatic L-amino acid decarboxylase.
MedLine Citation:
PMID:  17194446     Owner:  NLM     Status:  MEDLINE    
Autoimmune polyendocrine syndrome type I (APS I) is a rare hereditary condition considered a model disease for organ specific autoimmunity. A wide range of autoantibodies targeting antigens present in the affected organs have been identified. Autoantibodies against aromatic L-amino acid decarboxylase (AADC) are present in about 50% of APS I patients. In order to increase our understanding of autoantibody specificity in APS I, the aim of the present study was to localize target regions on AADC recognized by sera from APS I patients. Using several complementing strategies, we have shown that autoantibodies against AADC mainly recognize conformational epitopes. The major antigenic determinants were detected N-terminally to amino acid residue 237. Replacement of amino acids 227-230 (ERDK) with alanine residues reduced the reactivity towards AADC by >80% in all patient sera tested, suggesting that amino acids 227-230 are an important part of an immunodominant epitope.
Eirik Bratland; Anette S Bøe Wolff; Jan Haavik; Olle Kämpe; Filip Sköldberg; Jaakko Perheentupa; Geir Bredholt; Per M Knappskog; Eystein S Husebye
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-22
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  353     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-12     Completed Date:  2007-03-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  692-8     Citation Subset:  IM    
Department of Medicine, Haukeland University Hospital, N-5021 Bergen, Norway.
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MeSH Terms
Aromatic-L-Amino-Acid Decarboxylases / genetics,  immunology*
Carboxy-Lyases / genetics,  immunology
Epitope Mapping / methods*
Models, Molecular
Mutagenesis, Site-Directed
Polyendocrinopathies, Autoimmune / enzymology,  immunology
Recombinant Fusion Proteins / immunology
Reg. No./Substance:
0/Autoantibodies; 0/Recombinant Fusion Proteins; EC 4.1.1.-/Carboxy-Lyases; EC Decarboxylases; EC decarboxylase

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