Document Detail


Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein.
MedLine Citation:
PMID:  1371027     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human papillomavirus (HPV) E1--E4 protein is the only nonstructural late protein encoded by the virus. We have isolated three hybridomas producing monoclonal antibodies to the E1--E4 protein of HPV16, which is the HPV type most frequently associated with cervical cancer. The three antibodies (TVG 401, 402, and 403) detect adjacent epitopes within the major seroreactive region of the molecule and show no reactivity against the E4 proteins of HPV1, HPV2, HPV4, or HPV6. The E1--E4 protein migrates as a 10K species on SDS-gel electrophoresis and forms cytoplasmic inclusion granules in infected cells in vitro similar in appearance to those produced by HPV1 in benign warts. In naturally occurring HPV16-induced tumors the E1--E4 protein was detected in the cytoplasm of cells in the upper layers of the lesion in areas in which HPV16 DNA replication was occurring, as determined by in situ hybridization. Although the epitopes recognized by these monoclonal antibodies survive brief fixation in 5% formaldehyde, reactivity was destroyed by prolonged fixation. These monoclonal antibodies represent the first against HPV16 E1--E4 and should complement those already available to E7 and L1 for the screening of frozen sections of clinical biopsies and will be of value in monitoring the progression of HPV infection from benign lesions to invasive cancer.
Authors:
J Doorbar; S Ely; N Coleman; M Hibma; D H Davies; L Crawford
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Virology     Volume:  187     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1992 Mar 
Date Detail:
Created Date:  1992-03-12     Completed Date:  1992-03-12     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  353-9     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Cambridge, England.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Monoclonal / immunology
Base Sequence
Blotting, Western
Epitopes / immunology
Female
Fluorescent Antibody Technique
Humans
Hybridomas
Molecular Sequence Data
Oncogene Proteins, Fusion / immunology*
Oncogene Proteins, Viral / immunology*
Papillomaviridae / immunology*
Protein-Tyrosine Kinases / immunology*
Tumor Virus Infections / diagnosis*
Viral Proteins*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Epitopes; 0/Oncogene Proteins, Fusion; 0/Oncogene Proteins, Viral; 0/Viral Proteins; 0/oncogene protein E1--E4, Human papillomavirus type 16; 0/oncogene protein E4, human papilloma virus type 1; EC 2.7.10.1/Protein-Tyrosine Kinases

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