Document Detail


Epithelioid cell histiocytoma--histogenetic and kinetics analysis of dermal microvascular unit dendritic cell subpopulations.
MedLine Citation:
PMID:  12859738     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Epithelioid cell histiocytoma (ECH), also known as epithelioid fibrous histiocytoma, is a peculiar dermal tumor, which can mimic melanocytic, vascular, epithelial, or other histiocytic lesions. Thought to arise from dermal dendrocytes, most ECH contain approximately 50% FXIIIa+ histiocytic dendrocytes, but not all lesional cells express FXIIIa. A putative fibroblastic component has not been characterized. METHODS: We analyzed the differentiation and cell kinetics of dermal microvascular unit cells in 12 previously reported ECH using antibodies to FXIIIa, CD68 (KP1), CD34, CD117, CD31, smooth muscle actin, collagen type 1 aminopropeptide, and MIB-1, using single and double immunostains. RESULTS: In ECH, many variably sized CD34/CD31+ tumor vessels with actin+ myopericytes were surrounded by epithelioid-to-dendritic cells of three types. About 5-80% were dendritic histiocytes that expressed FXIIIa but not CD31 or KP1. Fibroblasts, in some cases showing mild nuclear pleomorphism, were usually collagen type 1+, but CD34 and actin- in 11/12 cases. One 'early' ECH had 40% CD34+ epithelioid cells, admixed with 50% FXIIIa+ histiocytes. Most ECH had about 2-20% KP1+, CD117+ mast cells. Mast cell numbers increased with FXIIIa+ histiocyte numbers and the intensity of FXIIIa expression. MIB-1/FXIIIa double-labeling showed only rare cycling histiocytes, with numerous cycling fibroblasts and endothelial cells. CONCLUSIONS: Our findings support the impression that ECH is a vascular fibrous histiocytoma. The constituent cells appear to arise from the activation of resident microvascular CD34+ dermal fibroblasts and the accumulation of FXIIIa+ dendritic stromal assembly histiocytes. The CD34+ cells appear to differentiate toward collagenous fibrocytes in association with histiocytes and mast cells in forming collagenous stroma and vessels. ECH is a tumor composed of all requisite cell types consistent with the origin from the dermal microvascular unit.
Authors:
Jeffrey S Silverman; Earl J Glusac
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cutaneous pathology     Volume:  30     ISSN:  0303-6987     ISO Abbreviation:  J. Cutan. Pathol.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-07-15     Completed Date:  2004-03-17     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0425124     Medline TA:  J Cutan Pathol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  415-22     Citation Subset:  IM    
Affiliation:
Southside Hospital, Department of Pathology, Bay Shore, NY, USA. peptolab@hamptons.com
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / metabolism
Cell Count
Cell Transformation, Neoplastic / pathology*
Dendritic Cells / metabolism,  pathology*
Epithelioid Cells / metabolism,  pathology*
Factor XIII / metabolism
Histiocytoma, Benign Fibrous / metabolism,  pathology*
Humans
Immunoenzyme Techniques
Kinetics
Skin Neoplasms / metabolism,  pathology*
Tumor Markers, Biological / metabolism
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Tumor Markers, Biological; 104641-95-4/factor XIII subunit A; 9013-56-3/Factor XIII

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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