Document Detail

Epithelio-mesenchymal transition in a neoplastic ovarian epithelial hybrid cell line.
MedLine Citation:
PMID:  15157238     Owner:  NLM     Status:  MEDLINE    
A hybrid cell line, IOSE-Ov29, was created through fusion of cells from the human ovarian adenocarcinoma line OVCAR3 and the non-tumorigenic SV40 Tag-transfected human ovarian surface epithelial line IOSE-29. OVCAR3 cells exhibit a differentiated epithelial phenotype, whereas line IOSE-29 expresses mesenchymal characteristics that were acquired in culture by epithelio-mesenchymal transition. Microsatellite analysis, comparative genomic hybridization (CGH), and MFISH showed the genotype of the IOSE-Ov29 cells to contain components of both parent cell lines, but to be predominantly OVCAR3 derived. IOSE-Ov29 resembled OVCAR3 and differed from IOSE-29 as shown by its unlimited life span, tumorigenicity, epithelial morphology, keratin, occludin, E-cadherin and CA125 expression, increased expression of kinases of the PI3K pathway, and loss of cGMP-dependent protein kinase expression. IOSE-29-derived properties included SV40 Tag expression, growth inhibition by activin, collagen type III secretion, increased adhesion and spreading on tissue culture plastic, and increased growth rate. Proliferation of all three lines was stimulated by FSH and ATP and inhibited by GnRH I and GnRH II. Interestingly, IOSE-Ov29 was more anchorage independent than either parent line and was the only line that invaded Matrigel in Boyden chambers and formed invasive branches in collagen gels. The results indicate that IOSE-Ov29 is an IOSE-29/OVCAR3 hybrid, which differs from both parent lines genetically and phenotypically. Unexpectedly, fusion with the non-tumorigenic IOSE-29 cells enhanced malignancy-associated characteristics of OVCAR3, presumably as a result of the expression of IOSE-29-derived mesenchymal properties that are usually acquired by carcinoma cells through epithelio-mesenchymal transition during metastatic progression.
Sarah L Maines-Bandiera; David Huntsman; Valia S Lestou; Wen-Lin Kuo; Peter C K Leung; R Douglas Horsman; Alice S T Wong; Michelle M M Woo; Keith K C Choi; Calvin D Roskelley; Nelly Auersperg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  72     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-05-25     Completed Date:  2005-01-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  150-61     Citation Subset:  IM    
Department of Obstetrics and Gynaecology, University of British Columbia, BC Women's Hospital, 4490 Oak Street, Vancouver, BC V6H 3V5, Canada.
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MeSH Terms
Adenocarcinoma / genetics,  metabolism,  pathology
Antigens, Polyomavirus Transforming / analysis,  genetics,  metabolism
Cadherins / analysis,  genetics,  metabolism
Carcinoma / genetics,  metabolism,  pathology*
Cell Line, Tumor*
Cell Nucleus / chemistry,  metabolism
Cell Transformation, Neoplastic*
Collagen Type III / analysis,  genetics,  metabolism
Culture Techniques
Epithelial Cells / metabolism,  pathology
Gene Expression Profiling
Hybrid Cells
Mesoderm / metabolism,  pathology
Oligonucleotide Array Sequence Analysis
Ovarian Neoplasms / genetics,  metabolism,  pathology*
Trinucleotide Repeats / genetics
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/Cadherins; 0/Collagen Type III

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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