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Epithelial tight junction alterations in nasal polyposis.
MedLine Citation:
PMID:  22287308     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVE: To explore alterations in expression of tight junction proteins (TJPs) in nasal polyposis and in respiratory epithelium under inflammatory conditions. Our hypothesis is that exposure of nasal and respiratory epithelium to inflammatory cytokines results in the altered expression of specific TJPs.
METHODS: Human sinonasal mucosa (3 nasal polyp specimens and 3 nonpolypoid controls) were stained with immunofluorescent markers specific for TJPs claudin-1 and occludin and examined with confocal scanning laser microscopy. A complementary in vitro experiment involving exposure of cultured human bronchial epithelium to interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) was also performed. Alterations in claudin-1 and occludin were localized by immunofluorescence labeling and confocal microscopy and quantified by western blotting.
RESULTS: Nasal polyp epithelium from human tissue specimens had reduced claudin-1 expression along the basal aspect of the mucosal layer, whereas occludin expression was reduced in the apical and basal epithelial zones. In vitro experiments demonstrated stable or increased TJP expression after 24 hours of cytokine exposure (43% increase for claudin-1, 9% increase for occludin). However, a reduction in TJP expression was observed after 72 hours of cytokine exposure (18% reduction for claudin-1, and 43% reduction for occludin).
CONCLUSION: Nasal polyposis is associated with epithelial TJP alterations. Further, the expression of TJPs in a model of inflamed respiratory mucosa is reduced in a similar fashion. Research on the histopathology of other epithelial inflammatory disorders suggests TJP alterations contribute to a self-perpetuating inflammatory state. Findings of this preliminary study support a similar process in nasal polyposis. © 2011 ARS-AAOA, LLC.
Authors:
G Aaron Rogers; Kyle Den Beste; Charles A Parkos; Asma Nusrat; John M Delgaudio; Sarah K Wise
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International forum of allergy & rhinology     Volume:  1     ISSN:  2042-6984     ISO Abbreviation:  Int Forum Allergy Rhinol     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2012-01-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101550261     Medline TA:  Int Forum Allergy Rhinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  50-4     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 American Rhinologic Society-American Academy of Otolaryngic Allergy, LLC.
Affiliation:
Department of Otolaryngology, Emory University, Atlanta, GA.
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