Document Detail

Epithelial-interstitial cell interactions in fetal rat lung development accelerated by steroids.
MedLine Citation:
PMID:  3736019     Owner:  NLM     Status:  MEDLINE    
Direct cell to cell contact has been suggested as the means whereby fibroblast factors influence epithelial cell differentiation in the developing lung. To obtain further evidence for this hypothesis, the role of epithelial-mesenchymal cell contacts is now examined when lung development is accelerated after steroid injection. Male and female rat fetuses were studied from day 17 to day 22 of gestation. The fetuses were from mothers injected with 2 mg/kg of dexamethasone at 2 days before death and [3H]thymidine at 1 hour before death. Steroid injected during the rapid growth phase reduced total DNA in fetal lung, and from autoradiographs, the effect was most noticeable in epithelial cells. Differentiation began sooner after steroid; more epithelial cells contained lamellar bodies and disaturated phosphatidylcholine levels were higher. From quantitative ultrastructural studies, these changes in phospholipid correlated with an increased frequency of epithelial-interstitial cell contacts. Steroid treatment did not abolish the sex-related difference seen in controls; both males and females showed increases in cell to cell contacts and in lipid synthesis in the lung. The results demonstrate that acceleration of epithelial cell maturation with increased surfactant synthesis is associated with increased epithelial-interstitial cell contacts. This supports the concept that regulatory messages from the fetal fibroblast are passed directly to specific epithelial cells to initiate surfactant synthesis.
I Y Adamson; G M King
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  55     ISSN:  0023-6837     ISO Abbreviation:  Lab. Invest.     Publication Date:  1986 Aug 
Date Detail:
Created Date:  1986-09-17     Completed Date:  1986-09-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  145-52     Citation Subset:  IM    
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MeSH Terms
Cell Differentiation / drug effects
Dexamethasone / pharmacology*
Epithelial Cells
Fetus / drug effects*
Injections, Intraperitoneal
Lung / cytology*
Microscopy, Electron
Phosphatidylcholines / analysis
Rats, Inbred Strains
Reg. No./Substance:
0/Phosphatidylcholines; 0/lecithins, disaturated; 50-02-2/Dexamethasone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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