| Epithelial expression of matrix metalloproteinase-26 is elevated at mid-cycle in the human endometrium. | |
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MedLine Citation:
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PMID: 12728020 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied MMP-26 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Tissue distribution and cycle variation was examined using in-situ hybridization, Northern blot analyis and real time PCR. The probes for Northern blot analysis and real time PCR recognized non-overlapping sequences. MMP-26 was localized exclusively in epithelial cells of both glands and the luminal surface. Expression increased during the proliferative phase to a maximum at mid-cycle, then decreased to non-detectable levels in the late secretory and menstrual phases. Expression of MMP-26 mRNA in endometrial tissue explants in vitro required stimulation with both estradiol and progesterone. The tissue content of c-jun mRNA was assayed, since c-jun, as part of the enhancer complex AP-1, may be involved in regulation of MMP-26 gene transcription. The pattern of c-jun expression over the menstrual cycle was similar to that of MMP-26. Epithelial expression in the peri- and post-ovulatory stages of the menstrual cycle suggests the involvement of MMP-26 in reproductive processes. |
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Authors:
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R Pilka; C Whatling; H Domanski; S Hansson; P Eriksson; B Casslén |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular human reproduction Volume: 9 ISSN: 1360-9947 ISO Abbreviation: Mol. Hum. Reprod. Publication Date: 2003 May |
Date Detail:
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Created Date: 2003-05-02 Completed Date: 2004-05-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9513710 Medline TA: Mol Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 271-7 Citation Subset: IM |
Affiliation:
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Department of Obstetrics & Gynecology, University Hospital, Lund, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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biosynthesis,
genetics Endometrium / enzymology* Epithelium / enzymology Female Gene Expression Regulation / physiology* Humans Matrix Metalloproteinases / biosynthesis, genetics* Matrix Metalloproteinases, Secreted Menstrual Cycle / metabolism* Organ Specificity Proto-Oncogene Proteins c-jun / biosynthesis, genetics RNA, Messenger / metabolism Transcription Factor AP-1 / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 0/Transcription Factor AP-1; EC 3.4.24.-/MMP26 protein, human; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.24.-/Matrix Metalloproteinases, Secreted |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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