Document Detail


Epithelial cells retain junctions during mitosis.
MedLine Citation:
PMID:  7685036     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has long been known that cells show reduced cell-substratum adhesion during mitosis in tissue culture, but it is not generally known whether cell-cell adhesion is also reduced. Epithelial cells, both in culture and in tissues, are linked together by several different types of intercellular junctions. Are these junctions disassembled when epithelial cells divide? Cultured epithelial cells were fluorescently stained for desmosomes, tight junctions and zonulae adherentes, and large numbers of dividing cells examined by light microscopy. The results suggested that all three types of intercellular junctions were retained throughout cell division and no evidence for internalization of junctions was obtained. The persistence of intercellular junctions by cultured cells during division was confirmed by electron microscopy. In order to determine whether intercellular junctions were similarly retained by dividing cells in tissues, human colonic mucosal crypt cells and basal keratinocytes were studied by electron microscopy. Both cell types retained intercellular junctions during division. Dividing basal keratinocytes also possessed hemidesmosomal contact with the basement membrane. It is suggested that retention of cellular junctions during division is important for maintenance of tissue integrity and organization.
Authors:
J Baker; D Garrod
Related Documents :
19719646 - Factors influencing the detection of bacterial cells using fluorescence in situ hybridi...
9462296 - Effects of sexual maturation and gonadal steroids on the localization of igg-, igm- and...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  104 ( Pt 2)     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-07-06     Completed Date:  1993-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  415-25     Citation Subset:  IM    
Affiliation:
Cancer Research Campaign, Medical Oncology Unit, University of Southampton, Southampton General Hospital, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actins / analysis
Animals
Cattle
Cell Adhesion
Cell Line / chemistry
Cells, Cultured / chemistry
Cytoskeletal Proteins / analysis
Desmoplakins
Desmosomes / chemistry,  ultrastructure
Dogs
Epithelium / ultrastructure
Humans
Intercellular Junctions / ultrastructure*
Intestinal Mucosa / cytology,  ultrastructure
Microscopy, Electron
Mitosis*
Skin / cytology,  ultrastructure
Staining and Labeling
Chemical
Reg. No./Substance:
0/Actins; 0/Cytoskeletal Proteins; 0/Desmoplakins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Induction of tenascin in cancer cells by interactions with embryonic mesenchyme mediated by a diffus...
Next Document:  Biological activity of recombinant human myelin basic protein.