Document Detail


Epithelial cell invasion by bovine septicemic Escherichia coli.
MedLine Citation:
PMID:  7903284     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Little is known regarding the pathogenesis of Escherichia coli-induced septicemic colibacillosis of calves. To understand the mechanism by which these strains penetrate the intestinal epithelium and gain access to the bloodstream, we examined the potential of bovine septicemic E. coli to invade cultured epithelial cells. By using a gentamicin survival assay, we demonstrated bacterial invasion of Madin-Darby canine kidney (MDCK) cells. Transcytosis of polarized MDCK cell monolayers was also observed, but only when bacteria were added to the basolateral surface. Electron microscopy confirmed the presence of intracellular organisms which appeared to be within membrane-bound vacuoles. The bovine septicemic isolate used in this study expressed the fimbrial adhesion CS31A. To examine the role of CS31A-mediated adherence in invasion and transcytosis of MDCK cell monolayers, a CS31A-deficient mutant was constructed by suicide vector-mediated insertional mutagenesis. Although nonadherent, the mutant showed a level of invasion similar to that of the wild-type parent. E. coli DH5 alpha carrying the cloned CS31A determinant was noninvasive. These findings suggest that expression of CS31A is neither required nor sufficient to mediate invasion.
Authors:
M J Korth; J C Lara; S L Moseley
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  62     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-01-27     Completed Date:  1994-01-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  41-7     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Washington, Seattle 98195.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Adhesion
Cattle
Cell Line
Cell Polarity
Cells, Cultured
Dogs
Epithelium / microbiology
Escherichia coli / pathogenicity*
Fimbriae, Bacterial / metabolism
Genes, Bacterial
Microscopy, Electron
Mutagenesis, Insertional
Sepsis / microbiology*
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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