| Epithelial cell differentiation pathways in the human prostate: identification of intermediate phenotypes by keratin expression. | |
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MedLine Citation:
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PMID: 11156695 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The prostate grows slowly throughout adult life, leading to benign prostatic hyperplasia (BPH), which often results in urethral obstruction in later years. The symptoms of BPH are the second most common reason for surgery in men over 65. The aim of this study was to determine the relationship between cell proliferation and cell differentiation in BPH tissue. Using multiple antibodies, simultaneously detected with different fluorophore-conjugated secondary antibodies, several subpopulations of epithelial cells were detected. In addition to K14, basal cells also expressed keratins 15, 17, and 19 in various combinations, and some of the luminal cells also expressed K19 together with K8 and K18. Co-staining for cytokeratins and Ki-67 indicated that 44% of proliferative cells expressed K14 and 36% K19, although the difference was not statistically significant. This report provides a detailed description of the relationship between keratin expression and cell proliferation in the prostate and indicates that K19-positive cells form the link between the basal and luminal layers of the epithelium. (J Histochem Cytochem 49:271-278, 2001) |
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Authors:
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D L Hudson; A T Guy; P Fry; M J O'Hare; F M Watt; J R Masters |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 49 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 2001 Feb |
Date Detail:
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Created Date: 2001-01-26 Completed Date: 2001-02-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: United States |
Other Details:
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Languages: eng Pagination: 271-8 Citation Subset: IM |
Affiliation:
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Institute of Urology and Nephrology, Research Laboratories, University College London Medical School, UK. d.hudson@ucl.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Antibodies Cell Compartmentation Cell Differentiation Cell Division Epithelial Cells / metabolism, pathology* Humans Immunohistochemistry Keratins / immunology, metabolism* Male Middle Aged Phenotype Prostate / pathology* Prostatic Hyperplasia / pathology |
| Grant Support | |
ID/Acronym/Agency:
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AG14960-02/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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