| Epithelial polarity proteins regulate Drosophila tracheal tube size in parallel to the luminal matrix pathway. | |
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MedLine Citation:
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PMID: 20022244 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Regulation of epithelial tube size is critical for organ function. However, the mechanisms of tube size control remain poorly understood. In the Drosophila trachea, tube dimensions are regulated by a luminal extracellular matrix (ECM). ECM organization requires apical (luminal) secretion of the protein Vermiform (Verm), which depends on the basolateral septate junction (SJ). Here, we show that apical and basolateral epithelial polarity proteins interact to control tracheal tube size independently of the Verm pathway. Mutations in yurt (yrt) and scribble (scrib), which encode SJ-associated polarity proteins, cause an expansion of tracheal tubes but do not disrupt Verm secretion. Reducing activity of the apical polarity protein Crumbs (Crb) suppresses the length defects in yrt but not scrib mutants, suggesting that Yrt acts by negatively regulating Crb. Conversely, Crb overexpression increases tracheal tube dimensions. Reducing crb dosage also rescues tracheal size defects caused by mutations in coracle (cora), which encodes an SJ-associated polarity protein. In addition, crb mutations suppress cora length defects without restoring Verm secretion. Together, these data indicate that Yrt, Cora, Crb, and Scrib operate independently of the Verm pathway. Our data support a model in which Cora and Yrt act through Crb to regulate epithelial tube size. |
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Authors:
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Patrick Laprise; Sarah M Paul; Jim Boulanger; Renée M Robbins; Greg J Beitel; Ulrich Tepass |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Current biology : CB Volume: 20 ISSN: 1879-0445 ISO Abbreviation: Curr. Biol. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-02-15 Completed Date: 2010-05-25 Revised Date: 2011-09-19 |
Medline Journal Info:
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Nlm Unique ID: 9107782 Medline TA: Curr Biol Country: England |
Other Details:
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Languages: eng Pagination: 55-61 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Laval University Cancer Research Center, CRCHUQ-Hôtel-Dieu de Québec, 9 McMahon, Québec, QC G1R 2J6, Canada. patrick.laprise@crhdq.ulaval.ca |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Body Patterning / genetics, physiology Drosophila / embryology*, genetics, physiology* Drosophila Proteins / genetics, physiology* Extracellular Matrix / physiology Genes, Insect Membrane Proteins / genetics, physiology Models, Biological Mutation Organ Size Trachea / embryology* Tumor Suppressor Proteins / genetics, physiology |
| Grant Support | |
ID/Acronym/Agency:
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5 T32 HL076139-0/HL/NHLBI NIH HHS; R01 GM069540/GM/NIGMS NIH HHS; R01 GM069540-05/GM/NIGMS NIH HHS; //Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/Drosophila Proteins; 0/Membrane Proteins; 0/Tumor Suppressor Proteins; 0/Yurt protein, Drosophila; 0/cora protein, Drosophila; 0/crumbs protein, Drosophila; 0/scribbled protein, Drosophila |
| Comments/Corrections | |
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