| Epithelial mesenchymal transition traits in human breast cancer cell lines parallel the CD44(hi/)CD24 (lo/-) stem cell phenotype in human breast cancer. | |
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MedLine Citation:
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PMID: 20521089 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We review here the recently emerging relationship between epithelial-mesenchymal transition (EMT) and breast cancer stem cells (BCSC), and provide analyses of published data on human breast cancer cell lines, supporting their utility as a model for the EMT/BCSC state. Genome-wide transcriptional profiling of these cell lines has confirmed the existence of a subgroup with mesenchymal tendencies and enhanced invasive properties ('Basal B'/Mesenchymal), distinct from subgroups with either predominantly luminal ('Luminal') or mixed basal/luminal ('Basal A') features (Neve et al. Cancer Cell, 2006). A literature-derived EMT gene signature has shown specific enrichment within the Basal B subgroup of cell lines, consistent with their over-expression of various EMT transcriptional drivers. Basal B cell lines are found to resemble BCSC, being CD44(high)CD24(low). Moreover, gene products that distinguish Basal B from Basal A and Luminal cell lines (Basal B Discriminators) showed close concordance with those that define BCSC isolated from clinical material, as reported by Shipitsin et al. (Cancer Cell, 2007). CD24 mRNA levels varied across Basal B cell lines, correlating with other Basal B Discriminators. Many gene products correlating with CD24 status in Basal B cell lines were also differentially expressed in isolated BCSC. These findings confirm and extend the importance of the cellular product of the EMT with Basal B cell lines, and illustrate the value of analysing these cell lines for new leads that may improve breast cancer outcomes. Gene products specific to Basal B cell lines may serve as tools for the detection, quantification, and analysis of BCSC/EMT attributes. |
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Authors:
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Tony Blick; Honor Hugo; Edwin Widodo; Mark Waltham; Cletus Pinto; Sendurai A Mani; Robert A Weinberg; Richard M Neve; Marc E Lenburg; Erik W Thompson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review Date: 2010-06-04 |
Journal Detail:
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Title: Journal of mammary gland biology and neoplasia Volume: 15 ISSN: 1573-7039 ISO Abbreviation: J Mammary Gland Biol Neoplasia Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-16 Completed Date: 2010-10-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9601804 Medline TA: J Mammary Gland Biol Neoplasia Country: United States |
Other Details:
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Languages: eng Pagination: 235-52 Citation Subset: IM |
Affiliation:
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Invasion and Metastasis Unit, St. Vincent's Institute, 9 Princes St, Fitzroy, Melbourne 3065, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD24
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metabolism* Antigens, CD44 / metabolism* Antineoplastic Agents / pharmacology, therapeutic use Breast Neoplasms / drug therapy, metabolism, physiopathology* Cell Dedifferentiation Cell Line, Tumor Cell Transdifferentiation* Epithelial Cells / physiology* Female Humans Mesenchymal Stem Cells / drug effects, physiology* Neoplastic Stem Cells / drug effects, physiology* Phenotype |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD24; 0/Antigens, CD44; 0/Antineoplastic Agents; 0/CD24 protein, human; 0/CD44 protein, human |
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