Document Detail


Epithelial mesenchymal transition traits in human breast cancer cell lines parallel the CD44(hi/)CD24 (lo/-) stem cell phenotype in human breast cancer.
MedLine Citation:
PMID:  20521089     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We review here the recently emerging relationship between epithelial-mesenchymal transition (EMT) and breast cancer stem cells (BCSC), and provide analyses of published data on human breast cancer cell lines, supporting their utility as a model for the EMT/BCSC state. Genome-wide transcriptional profiling of these cell lines has confirmed the existence of a subgroup with mesenchymal tendencies and enhanced invasive properties ('Basal B'/Mesenchymal), distinct from subgroups with either predominantly luminal ('Luminal') or mixed basal/luminal ('Basal A') features (Neve et al. Cancer Cell, 2006). A literature-derived EMT gene signature has shown specific enrichment within the Basal B subgroup of cell lines, consistent with their over-expression of various EMT transcriptional drivers. Basal B cell lines are found to resemble BCSC, being CD44(high)CD24(low). Moreover, gene products that distinguish Basal B from Basal A and Luminal cell lines (Basal B Discriminators) showed close concordance with those that define BCSC isolated from clinical material, as reported by Shipitsin et al. (Cancer Cell, 2007). CD24 mRNA levels varied across Basal B cell lines, correlating with other Basal B Discriminators. Many gene products correlating with CD24 status in Basal B cell lines were also differentially expressed in isolated BCSC. These findings confirm and extend the importance of the cellular product of the EMT with Basal B cell lines, and illustrate the value of analysing these cell lines for new leads that may improve breast cancer outcomes. Gene products specific to Basal B cell lines may serve as tools for the detection, quantification, and analysis of BCSC/EMT attributes.
Authors:
Tony Blick; Honor Hugo; Edwin Widodo; Mark Waltham; Cletus Pinto; Sendurai A Mani; Robert A Weinberg; Richard M Neve; Marc E Lenburg; Erik W Thompson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review     Date:  2010-06-04
Journal Detail:
Title:  Journal of mammary gland biology and neoplasia     Volume:  15     ISSN:  1573-7039     ISO Abbreviation:  J Mammary Gland Biol Neoplasia     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-16     Completed Date:  2010-10-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9601804     Medline TA:  J Mammary Gland Biol Neoplasia     Country:  United States    
Other Details:
Languages:  eng     Pagination:  235-52     Citation Subset:  IM    
Affiliation:
Invasion and Metastasis Unit, St. Vincent's Institute, 9 Princes St, Fitzroy, Melbourne 3065, Australia.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD24 / metabolism*
Antigens, CD44 / metabolism*
Antineoplastic Agents / pharmacology,  therapeutic use
Breast Neoplasms / drug therapy,  metabolism,  physiopathology*
Cell Dedifferentiation
Cell Line, Tumor
Cell Transdifferentiation*
Epithelial Cells / physiology*
Female
Humans
Mesenchymal Stem Cells / drug effects,  physiology*
Neoplastic Stem Cells / drug effects,  physiology*
Phenotype
Chemical
Reg. No./Substance:
0/Antigens, CD24; 0/Antigens, CD44; 0/Antineoplastic Agents; 0/CD24 protein, human; 0/CD44 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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