Document Detail


Epithelial cells lining salivary gland ducts are early target cells of severe acute respiratory syndrome coronavirus infection in the upper respiratory tracts of rhesus macaques.
MedLine Citation:
PMID:  21289121     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The shedding of severe acute respiratory syndrome coronavirus (SARS-CoV) into saliva droplets plays a critical role in viral transmission. The source of high viral loads in saliva, however, remains elusive. Here we investigate the early target cells of infection in the entire array of respiratory tissues in Chinese macaques after intranasal inoculations with a single-cycle pseudotyped virus and a pathogenic SARS-CoV. We found that angiotensin-converting enzyme 2-positive (ACE2(+)) cells were widely distributed in the upper respiratory tract, and ACE2(+) epithelial cells lining salivary gland ducts were the early target cells productively infected. Our findings also have implications for SARS-CoV early diagnosis and prevention.
Authors:
Li Liu; Qiang Wei; Xavier Alvarez; Haibo Wang; Yanhua Du; Hua Zhu; Hong Jiang; Jingying Zhou; Pokman Lam; Linqi Zhang; Andrew Lackner; Chuan Qin; Zhiwei Chen
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-02-02
Journal Detail:
Title:  Journal of virology     Volume:  85     ISSN:  1098-5514     ISO Abbreviation:  J. Virol.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-22     Completed Date:  2011-05-18     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4025-30     Citation Subset:  IM    
Affiliation:
AIDS Institute, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Epithelial Cells / virology*
Immunohistochemistry
Macaca mulatta
Microscopy
Peptidyl-Dipeptidase A / analysis
Receptors, Virus / analysis
SARS Virus / isolation & purification*,  pathogenicity
Salivary Ducts / virology*
Severe Acute Respiratory Syndrome / veterinary*,  virology*
Viral Load
Grant Support
ID/Acronym/Agency:
R01 HL080211-02/HL/NHLBI NIH HHS; RR00164/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Virus; EC 3.4.15.1/Peptidyl-Dipeptidase A; EC 3.4.17.-/angiotensin converting enzyme 2
Comments/Corrections

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