Document Detail


Epistatic natural allelic variation reveals a function of AGAMOUS-LIKE6 in axillary bud formation in Arabidopsis.
MedLine Citation:
PMID:  22730404     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the Arabidopsis multiparent recombinant inbred line mapping population, a limited number of plants were detected that lacked axillary buds in most of the axils of the cauline (stem) leaves, but formed such buds in almost all rosette axils. Genetic analysis showed that polymorphisms in at least three loci together constitute this phenotype, which only occurs in late-flowering plants. Early flowering is epistatic to two of these loci, called REDUCED SHOOT BRANCHING1 (RSB1) and RSB2, which themselves do not affect flowering time. Map-based cloning and confirmation by transformation with genes from the region where RSB1 was identified by fine-mapping showed that a specific allele of AGAMOUS-Like6 from accession C24 conferred reduced branching in the cauline leaves. Site-directed mutagenesis in the Columbia allele revealed the causal amino acid substitution, which behaved as dominant negative, as was concluded from a loss-of-function mutation that showed the same phenotype in the late-flowering genetic background. This causal allele occurs at a frequency of 15% in the resequenced Arabidopsis thaliana accessions and correlated with reduced stem branching only in late-flowering accessions. The data show the importance of natural variation and epistatic interactions in revealing gene function.
Authors:
Xueqing Huang; Sigi Effgen; Rhonda Christiane Meyer; Klaus Theres; Maarten Koornneef
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Publication Detail:
Type:  Journal Article     Date:  2012-06-22
Journal Detail:
Title:  The Plant cell     Volume:  24     ISSN:  1532-298X     ISO Abbreviation:  Plant Cell     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-07-30     Completed Date:  2012-12-05     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2364-79     Citation Subset:  IM    
Affiliation:
Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/JX121859;  JX121966
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MeSH Terms
Descriptor/Qualifier:
Alleles
Amino Acid Substitution
Arabidopsis / genetics*,  growth & development*
Arabidopsis Proteins / genetics*,  metabolism
Cloning, Molecular
Epistasis, Genetic*
Gene Expression Regulation, Plant
Gene Frequency
Molecular Sequence Data
Mutagenesis, Site-Directed
Period Circadian Proteins / genetics*,  metabolism
Phenotype
Plant Leaves / genetics,  growth & development*
Plant Stems / genetics,  growth & development*
Polymorphism, Genetic
Quantitative Trait Loci
Chemical
Reg. No./Substance:
0/AGL6 protein, Arabidopsis; 0/Arabidopsis Proteins; 0/Period Circadian Proteins
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