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Episkin: An in vitro model for the evaluation of phototoxicity and sunscreen photoprotective properties.
MedLine Citation:
PMID:  20692984     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Exposure to UV radiation has been shown to induce cutaneous biological changes and chemical modifications of exogenous compounds. The application of sunscreen limits UV-induced cutaneous injury. Besides damages can also be elicited by some chemicals following UV irradiation (phototoxic agents). The effects of UVA and UVB irradiation have been studied in vitro on a reconstituted epidermis (EPISKIN) for cytotoxicity (MTT conversion test) and release of a proinflammatory mediator, interleukin 1alpha. When compared with irradiated human keratinocyte monolayers, the results obtained with EPISKIN showed the protective function of the stratum corneum to UVB. Topical application of a UVB sunscreen directly on EPISKIN stratum corneum showed a concentration-dependent relationship between the active ingredients and UVB-induced damages evaluated by both parameters. When chlorpromazine was tested on EPISKIN, an increase in cytotoxicity and release of interleukin 1alpha followed UVA irradiation. A non-cytotoxic dose of 10 J/cm(2) of UVA induced a 20-fold decrease in the chlorpromazine IC50. The chlorpromazine-induced inflammatory reaction was also reflected by a more intense release of interleukin 1alpha in the underlying media of EPISKIN. Thus this proinflammatory mediator seems to be relevant as a phototoxic marker. The EPISKIN model may be a useful tool in the study of UV-induced cutaneous damage. The design of this study represents an advance in non-animal test development and could be of great interest in the determination of phototoxic or photoprotective effects of compounds in vitro.
Authors:
C Cohen; K G Dossou; A Rougier; R Roguet
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  8     ISSN:  0887-2333     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  1994 Aug 
Date Detail:
Created Date:  2010-08-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  669-71     Citation Subset:  -    
Affiliation:
Laboratoires de Recherche de L'Oral, 1 av. E. Schueller 93600, Aulnay-sous-Bois, France.
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