Document Detail

Epigenetic status of the H19 locus in human oocytes following in vitro maturation.
MedLine Citation:
PMID:  16378710     Owner:  NLM     Status:  MEDLINE    
Imprinting is an epigenetic modification that is reprogrammed in the germ line and leads to the monoallelic expression of some genes. Imprinting involves DNA methylation. Maternal imprint is reset during oocyte growth and maturation. In vitro maturation (IVM) of oocytes may, therefore, interfere with imprint acquisition and/or maintenance. To evaluate if maturing human oocytes in vitro would be hazardous at the epigenetic level, we first determined the methylation profile of the H19 differentially methylated region (DMR). The methylation status of the H19 DMR seems particularly vulnerable to in vitro culture conditions. We analyzed oocytes at different stages of maturation following IVM, germinal vesicle (GV), metaphase I (MI), and metaphase II (MII), using the bisulfite mutagenesis technique. Our results indicated that the unmethylated specific maternal profile for the H19 DMR was stably established at the GV stage. The majority of MI-arrested oocytes exhibited an altered pattern of methylation, the CTCF-binding site being methylated in half of the DNA strands analyzed. Of the 20 MII oocytes analyzed, 15 showed the normal unmethylated maternal pattern, while 5 originating from two different patients exhibited a methylated pattern. These findings highlight the need for extended analysis on MII-rescued oocytes to appreciate the epigenetic safety of the IVM procedure, before it becomes a routine and practical assisted reproductive procedure.
Nada Borghol; Jacqueline Lornage; Thierry Blachère; Anne Sophie Garret; Annick Lefèvre
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-27
Journal Detail:
Title:  Genomics     Volume:  87     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-13     Completed Date:  2006-04-28     Revised Date:  2011-10-07    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  417-26     Citation Subset:  IM    
INSERM U 418/INRA UA 953, Hopital Debrousse, 29 rue Soeur Bouvier, 69322 Lyon Cedex 05, France.
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MeSH Terms
Binding Sites / genetics
CpG Islands / genetics
DNA / genetics,  metabolism
DNA Methylation
DNA Restriction Enzymes / metabolism
DNA-Binding Proteins / metabolism
Epigenesis, Genetic
Insulin-Like Growth Factor II / genetics
Oocytes / growth & development,  metabolism*
RNA, Untranslated / genetics*
Repressor Proteins / metabolism
Time Factors
Reg. No./Substance:
0/CCCTC-binding factor; 0/DNA-Binding Proteins; 0/H19 long non-coding RNA; 0/RNA, Untranslated; 0/Repressor Proteins; 67763-97-7/Insulin-Like Growth Factor II; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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