Document Detail


Epigenetic silencing of the WNT antagonist DICKKOPF-1 in cervical cancer cell lines.
MedLine Citation:
PMID:  18377964     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Our study was designed to demonstrate that DICKKOPF-1 (DKK-1), encoding a secreted Wnt antagonist, is transcriptionally repressed by epigenetic alterations in cervical carcinoma cell lines. METHODS: Methylation-specific PCR for 8 human cervical cancer cell lines and bisulfite sequencing for 4 cell lines exhibiting significant difference in methylation levels were used to determine CpG-island methylation status at the 5'-end region of DKK-1. The chromatin immunoprecipitation assay was performed to determine whether HeLa cells recruit histone deacetylation for DKK-1 silencing. RESULTS: Two out of eight cervical cancer cell lines examined were found to be regulated by independent epigenetic inactivation mechanisms; promoter CpG hypermethylation constitutes a major epigenetic alteration in SNU-703, and histone deacetylation in HeLa cells. CONCLUSION: Our study suggests that cervical cancer cell lines exploit cell line-dependent, differential epigenetic mechanisms for DKK-1 silencing.
Authors:
Jaehyouk Lee; Young Soo Yoon; Jae Hoon Chung
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Publication Detail:
Type:  Journal Article     Date:  2008-04-02
Journal Detail:
Title:  Gynecologic oncology     Volume:  109     ISSN:  1095-6859     ISO Abbreviation:  Gynecol. Oncol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-05     Completed Date:  2008-06-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0365304     Medline TA:  Gynecol Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  270-4     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
CpG Islands / genetics
DNA Methylation
Epigenesis, Genetic*
Female
Gene Silencing*
Hela Cells
Histone Deacetylases / metabolism
Humans
Intercellular Signaling Peptides and Proteins / genetics*
Promoter Regions, Genetic
Uterine Cervical Neoplasms / enzymology,  genetics*
Wnt Proteins / antagonists & inhibitors*
Chemical
Reg. No./Substance:
0/DKK1 protein, human; 0/Intercellular Signaling Peptides and Proteins; 0/Wnt Proteins; EC 3.5.1.98/Histone Deacetylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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