Document Detail


Epigenetic inheritance through self-recruitment of the polycomb repressive complex 2.
MedLine Citation:
PMID:  19377285     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Maintenance of gene expression through epigenetic mechanisms such as DNA- and histone-methylation is essential for preserving cellular identity and function. Multiplication of eukaryotic cells requires that the DNA content of the cell is duplicated through replication, which is coupled to incorporation of de novo synthesized core histones into nucleosomal structures. One of the challenging questions in biology is to explain how the organism ensures that regulatory epigenetic marks, once established, are transferred from one cell generation to the next. Based on studies in our laboratory, we have recently proposed a model for how the methylated lysine 27 of histone H3 (H3K27) can be stably transmitted through the cell division cycle. We found that the Polycomb Repressive Complex 2 (PRC2), which is responsible for di- and trimethylation of H3K27 (H3K27me2/me3), binds to its own site of methylation. Moreover, our results suggested that maintenance of transcriptional repression by PRC2 requires the binding of the PRC2 complex to H3K27me3/me2. Based on these two key observations we propose that PRC2 is able to copy the mark from an old parental H3 molecule to a newly synthesized H3 molecule as DNA replication proceeds. In addition, our results support a model for how the H3K27me3 mark could be preserved in the interphase of the cell cycle, where other events such as histone exchange and demethylation could counteract PRC2 function. Here we discuss the implications of our results in further detail.
Authors:
Klaus H Hansen; Kristian Helin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-18
Journal Detail:
Title:  Epigenetics : official journal of the DNA Methylation Society     Volume:  4     ISSN:  1559-2308     ISO Abbreviation:  Epigenetics     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-05-21     Completed Date:  2009-08-04     Revised Date:  2010-08-06    
Medline Journal Info:
Nlm Unique ID:  101265293     Medline TA:  Epigenetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  133-8     Citation Subset:  IM    
Affiliation:
Biotech Research and Innovation Centre (BRIC), University of Copenhagen, Copenhagen, Denmark. klaus.hansen@bric.ku.dk
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MeSH Terms
Descriptor/Qualifier:
Animals
Drosophila melanogaster / genetics,  metabolism
Epigenesis, Genetic*
Evolution, Molecular
Humans
Lysine / metabolism*
Methylation
Protein Binding
Repressor Proteins / metabolism*
Chemical
Reg. No./Substance:
0/Repressor Proteins; 0/polycomb group proteins; 56-87-1/Lysine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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