Document Detail


Epigenetic inactivation of RASSF1A candidate tumor suppressor gene at 3p21.3 in brain tumors.
MedLine Citation:
PMID:  14586413     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human Ras association domain family 1A (RASSF1A) gene, recently isolated from the lung and breast tumor suppressor locus 3p21.3, is highly methylated in primary lung, breast, nasopharyngeal and other tumors, and re-expression of RASSF1A suppresses the growth of several types of cancer cells. Epigenetic inactivation of RASSF1A by promoter hypermethylation is also important in the development of several human cancers. The methylation status of the promoter region of RASSF1A was analysed in primary brain tumors and glioma cell lines by methylation-specific polymerase chain reaction. In primary brain tumors, 25 of 46 (54.3%) gliomas and five of five (100%) medulloblastomas showed RASSF1A methylation. In benign tumors, only one of 10 (10%) schwannomas and two of 12 (16.7%) meningiomas showed RASSF1A methylation. The RASSF1A promoter region was methylated in all four glioma cell lines. RASSF1A was re-expressed in all methylated cell lines after treatment with the demethylating agent 5-aza-2'-deoxycytidine. Methylation of the promoter CpG islands of the RASSF1A may play an important role in the pathogenesis of glioma and medulloblastoma.
Authors:
Keishi Horiguchi; Yoshio Tomizawa; Masahiko Tosaka; Shogo Ishiuchi; Hideyuki Kurihara; Masatomo Mori; Nobuhito Saito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncogene     Volume:  22     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-30     Completed Date:  2003-12-03     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  England    
Other Details:
Languages:  eng     Pagination:  7862-5     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Gunma University School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan. hkeishi@med.gunma-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Azacitidine / analogs & derivatives*,  pharmacology
Brain Neoplasms / genetics*
Cell Line, Tumor
Chromosomes, Human, Pair 3*
CpG Islands
DNA Methylation
Genes, Tumor Suppressor*
Glioma / genetics
Humans
Medulloblastoma / genetics
Neoplasm Proteins / genetics*
Promoter Regions, Genetic
Tumor Suppressor Proteins*
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 0/RASSF1 protein, human; 0/Tumor Suppressor Proteins; 320-67-2/Azacitidine; 776B62CQ27/decitabine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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