| Epigenetic and immunohistochemical characterization of the Clusterin gene in ovarian tumors. | |
| | |
MedLine Citation:
|
PMID: 23208459 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
PURPOSE: To characterize abnormal epigenetic changes and protein expression of the clusterin gene in a large series of ovarian malignant and borderline tumors. METHODS: Protein expression and promoter methylation of clusterin gene in 181 primary ovarian epithelial cancer, 40 borderline ovarian tumors, 54 ovarian cancer mesenteric metastasis, and 10 normal ovarian samples were analyzed by immunohistochemical staining and methylation-specific PCR. RESULTS: Overexpression of clusterin protein was frequently seen in various ovarian epithelial tumors, being detected in 102 of 181 (56 %) primary ovarian epithelial cancers, 21 of 37 (57 %) borderline ovarian tumors. Surprisingly, clusterin protein expression was significantly reduced in mesenteric metastasis (20 of 54; 37 % cases), as compared to primary ovarian carcinoma (p = 0.01). Overexpression of clusterin protein was significantly correlated with high-grade histology (p = 0.002) and high FIGO stages (p = 0.05). Clusterin promoter hypermethylation was detected in 24 of 181 (13 %) primary ovarian epithelial cancer, 8 of 54 (14 %) mesenteric metastasis, and 10 of 37 (27 %) borderline ovarian tumors. Overall, clusterin promoter hypermethylation was significantly correlated with decreased protein expression in these samples (p < 0.001). CONCLUSIONS: Increased clusterin expression is correlated with more aggressive biologic behavior in ovarian cancer. Promoter methylation of the clusterin gene can be readily detected, though at low frequencies, in ovarian epithelial tumors and is significantly associated with decreased protein expression of the gene. |
| | |
Authors:
|
Guofen Yang; Haihong Zhang; Yu Liu; Juan Zhou; Weipeng He; Charles M Quick; Dan Xie; Bruce R Smoller; Chun-Yang Fan |
Related Documents
:
|
23325349 - Antioxidant and anti-tumor activity of a polysaccharide from freshwater clam, corbicula... 23386689 - Hepatocyte growth factor sensitizes brain tumors to c-met kinase inhibition. 23440329 - Praf2 stimulates cell proliferation and migration and predicts poor prognosis in neurob... 23371049 - Timp-2 modulates cancer cell transcriptional profile and enhances e-cadherin/beta-caten... 23208009 - Characteristic magnifying narrow-band imaging features of colorectal tumors in each gro... 23364389 - Tumor-associated macrophage promotes tumor progression via stat3 signaling in hepatocel... 19822429 - Antigen p57/kip2 as a potential negative regulator of human astrocytoma growth. 9299269 - Primary endodermal sinus tumor of the vulva: a case report and review of the literature. 21940319 - Acetazolamide inhibits aquaporin-1 expression and colon cancer xenograft tumor growth. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-12-4 |
Journal Detail:
|
Title: Archives of gynecology and obstetrics Volume: - ISSN: 1432-0711 ISO Abbreviation: Arch. Gynecol. Obstet. Publication Date: 2012 Dec |
Date Detail:
|
Created Date: 2012-12-4 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8710213 Medline TA: Arch Gynecol Obstet Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Gynecology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Meta-analysis of the efficacy and safety of the application of adjuvant material in the repair of an...
Next Document: Sublingual versus vaginal misoprostol for cervical ripening before hysteroscopy: a randomized clinic...