Document Detail


Epigenetic disregulation induces cell growth retardation in primary cultured glial cells.
MedLine Citation:
PMID:  18558336     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Some epigenetic mechanisms, including DNA methylation and histone deacetylation, act as transcriptional repression signals. In this study, we examined whether DNA methylation dependent transcriptional control regulates glial cell growth. Primary cultured mouse cortical glial cells were treated with the DNA methylation inhibitor 5-aza-deoxycytidine (5adC) or the histone deacetylase inhibitor sodium valproate (VPA), which inhibits DNA-methylation-dependent transcriptional repression. 5adC significantly reduced methylated C level determined by reversed-phase high-performance liquid chromatography (HPLC), while VPA did not. Treatments with these inhibitors significantly reduced cell number determined by MTT assay after 48 h. Both 5adC and VPA showed little cellular toxicity observed by live and dead cell staining. In contrast, both 5adC and VPA induced an abnormality in the cell cycle. Cells treated with the inhibitors represented a significantly higher ratio in the G2+M-phase and 5adC-treated cells showed a significantly lower ratio in the S-phase. Regarding the in vivo effect, prenatal treatment with VPA, which is an autistic model in rodents, significantly reduced the brain/body weight ratio in early postnatal days. Our data indicate that DNA-methylation- and histone-deacetylation-dependent transcriptional control is crucial for the regulation of glial cell growth. Our data suggest that abnormalities of epigenetic transcriptional regulatory mechanisms in glial cells cause an abnormal brain size, which may in turn cause mental diseases.
Authors:
Kaoru Nagai; Takamitsu Natori; Toru Nishino; Fumiaki Kodaira
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  105     ISSN:  1347-4421     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-06-18     Completed Date:  2008-09-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  470-5     Citation Subset:  IM    
Affiliation:
Department of Epigenetic Medicine, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan. kaoru@yamanashi.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Proliferation / drug effects
Cells, Cultured
Cerebral Cortex / drug effects,  growth & development*
Epigenesis, Genetic / drug effects*
Female
Fetal Growth Retardation / chemically induced,  pathology*,  physiopathology*
Mice
Mice, Inbred C57BL
Neuroglia / pathology*,  physiology*
Valproic Acid
Chemical
Reg. No./Substance:
99-66-1/Valproic Acid

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