Document Detail

Epigenetic regulation of CXCR4 expression by the ocular microenvironment.
MedLine Citation:
PMID:  23188729     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Expression of the chemokine receptor CXCR4 by tumors is associated with metastatic migration and invasion of tumor cells. The importance of CXCR4 expression by uveal melanomas in metastasis to the liver was recently demonstrated when injection of CXCR4-negative uveal melanoma cells into mice resulted in reduced liver metastasis compared with CXCR4-positive uveal melanoma cells. Factors in the eye can induce downregulation of genes by epigenetic mechanisms. This study examined whether epigenetic regulation by the ocular environment induced downregulation of CXCR4 expression.
METHODS: LS174T colon cancer cells were injected in the anterior chamber (AC), subcutaneously (SC), or in the spleen capsule to induce liver metastasis in immune-deficient mice. CXCR4 gene transcription was analyzed by RT-PCR, and protein expression was determined by flow cytometry. Methyltransferase and histone deacetylase activities were determined by ELISA. Treatment with either 5-Aza-2-deoxycytidine (5-Aza) or trichostatin A (TSA) was used to induce demethylation or inhibit histone deacetylases, respectively.
RESULTS: AC-derived LS174T cells showed lower CXCR4 gene expression compared with SC-, liver-derived, or wild-type tumor cells. AC-derived LS174T tumor cells expressed methyltransferase activity compared with SC-, liver-derived, and wild-type tumor cells. Deacetylase activity was elevated in AC-derived LS174T tumor cells compared with SC-derived, liver-derived, and wild-type tumor cells. Treatment of AC-derived LS174T tumor cells with 5-Aza upregulated CXCR4 expression. TSA treatment did not restore CXCR4 expression.
CONCLUSIONS: These studies demonstrate that ocular microenvironment factors induce methylation and downregulation of tumor CXCR4 expression.
Haochuan Li; Jerry Y Niederkorn; Leila Sadegh; Jessamee Mellon; Peter W Chen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-09
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  54     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-10     Completed Date:  2013-03-12     Revised Date:  2013-10-30    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  234-43     Citation Subset:  IM    
Department of Ophthalmology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390-9057, USA.
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MeSH Terms
Anterior Chamber / metabolism*,  pathology
Antimetabolites, Antineoplastic / pharmacology
Azacitidine / analogs & derivatives,  pharmacology
Cecum / metabolism,  pathology
Cell Line, Tumor
Epigenesis, Genetic*
Flow Cytometry
Gene Expression
Gene Expression Regulation, Neoplastic / physiology
Histone Deacetylase Inhibitors / administration & dosage
Histone Deacetylases / metabolism
Histones / metabolism
Hydroxamic Acids / administration & dosage
Injections, Subcutaneous
Lysine / metabolism
Mice, Inbred BALB C
Neoplasm Transplantation
Promoter Regions, Genetic
Receptors, CXCR4 / drug effects,  genetics*,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Spleen / metabolism,  pathology
Grant Support
Reg. No./Substance:
0/Antimetabolites, Antineoplastic; 0/CXCR4 protein, mouse; 0/Histone Deacetylase Inhibitors; 0/Histones; 0/Hydroxamic Acids; 0/Receptors, CXCR4; 320-67-2/Azacitidine; 3X2S926L3Z/trichostatin A; 56-87-1/Lysine; 776B62CQ27/decitabine; EC Deacetylases

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