Document Detail


Epigenetic marks define the lineage and differentiation potential of two distinct neural crest-derived intermediate odontogenic progenitor populations.
MedLine Citation:
PMID:  23379639     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Epigenetic mechanisms, such as histone modifications, play an active role in the differentiation and lineage commitment of mesenchymal stem cells. In the present study, epigenetic states and differentiation profiles of two odontogenic neural crest-derived intermediate progenitor populations were compared: dental pulp (DP) and dental follicle (DF). ChIP on chip assays revealed substantial H3K27me3-mediated repression of odontoblast lineage genes DSPP and dentin matrix protein 1 (DMP1) in DF cells, but not in DP cells. Mineralization inductive conditions caused steep increases of mineralization and patterning gene expression levels in DP cells when compared to DF cells. In contrast, mineralization induction resulted in a highly dynamic histone modification response in DF cells, while there was only a subdued effect in DP cells. Both DF and DP progenitors featured H3K4me3-active marks on the promoters of early mineralization genes RUNX2, MSX2, and DLX5, while OSX, IBSP, and BGLAP promoters were enriched for H3K9me3 or H3K27me3. Compared to DF cells, DP cells expressed higher levels of three pluripotency-associated genes, OCT4, NANOG, and SOX2. Finally, gene ontology comparison of bivalent marks unique for DP and DF cells highlighted cell-cell attachment genes in DP cells and neurogenesis genes in DF cells. In conclusion, the present study indicates that the DF intermediate odontogenic neural crest lineage is distinguished from its DP counterpart by epigenetic repression of DSPP and DMP1 genes and through dynamic histone enrichment responses to mineralization induction. Findings presented here highlight the crucial role of epigenetic regulatory mechanisms in the terminal differentiation of odontogenic neural crest lineages.
Authors:
Gokul Gopinathan; Antonia Kolokythas; Xianghong Luan; Thomas G H Diekwisch
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-03-15
Journal Detail:
Title:  Stem cells and development     Volume:  22     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-05-30     Completed Date:  2013-12-17     Revised Date:  2014-06-17    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1763-78     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Biological Markers / metabolism
Calcification, Physiologic / genetics
Cell Differentiation
Cell Lineage / genetics*
Child
Dental Pulp / cytology*,  metabolism
Dental Sac / cytology*,  metabolism
Epigenesis, Genetic*
Extracellular Matrix Proteins / genetics,  metabolism
Gene Expression Profiling
Gene Expression Regulation, Developmental
Humans
Jumonji Domain-Containing Histone Demethylases / genetics,  metabolism
Mesenchymal Stromal Cells / cytology*,  metabolism
Neural Crest / cytology*,  metabolism
Organ Specificity
Osteogenesis / genetics
Phosphoproteins / genetics,  metabolism
Promoter Regions, Genetic
Sialoglycoproteins / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
DE019463/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/DMP1 protein, human; 0/Extracellular Matrix Proteins; 0/Phosphoproteins; 0/Sialoglycoproteins; 0/dentin sialophosphoprotein; EC 1.14.11.-/Jumonji Domain-Containing Histone Demethylases; EC 1.14.11.-/KDM6B protein, human
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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