Document Detail

Epigallocatechin gallate's protective effect against MMP7 in recessive dystrophic epidermolysis bullosa patients.
MedLine Citation:
PMID:  17139264     Owner:  NLM     Status:  MEDLINE    
The analysis of phenotype-genotype correlations of patients suffering from recessive dystrophic epidermolysis bullosa (RDEB) evidenced intrafamilial and interfamilial phenotype variability occurring for the same mutation of COL7A1; this underscores the role of other genetics environmental factors in the expressivity of the disease. In this work, we checked whether matrilysin 1 (matrix metalloproteinase (MMP)7) could take part in the epidermal detachment in RDEB. Furthermore, we investigated epigallocatechin 3 gallate (EGCG) to determine whether it could inhibit matrilysin activities on collagen type VII and fibrillin 1 known to be associated with the dermo-epidermal junction. In this work, matrilysin 1 was detected in affected and unaffected skins of the three RDEB patients; furthermore, MMP7 was shown to degrade ex vivo on healthy normal skin collagen VII and fibrillin 1. Thus, we suspect that MMP7 could take an active part in the epidermal detachment occurring during RDEB. We evidenced that EGCG in in vitro as well as in ex vivo experiments was a good inhibitor of MMP7 and developed a good protection of collagen type VII and fibrillin 1 susceptible of being degraded by MMP7. We therefore propose that EGCG could be used beneficially in patients suffering from RDEB.
Sylvie Igondjo-Tchen Changotade; Antoine Assoumou; Farida Guéniche; Florence Fioretti; Sylvie Séguier; Yves de Prost; Christine Bodemer; Gaston Godeau; Karim Senni
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Publication Detail:
Type:  In Vitro; Journal Article     Date:  2006-11-30
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  127     ISSN:  1523-1747     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-16     Completed Date:  2007-04-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  821-8     Citation Subset:  IM    
Faculté de Chirurgie Dentaire de Montrouge, Université Paris, Montrouge, France.
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MeSH Terms
Antigens, CD / metabolism
Antigens, Differentiation, Myelomonocytic / metabolism
Blotting, Western
Catechin / analogs & derivatives*,  pharmacology
Collagen Type VII / metabolism
Elastic Tissue / pathology
Epidermolysis Bullosa Dystrophica / genetics,  pathology,  physiopathology*
Genes, Recessive*
Immunologic Techniques
Matrix Metalloproteinase 7 / antagonists & inhibitors*,  metabolism*
Microfilament Proteins / metabolism
Organ Culture Techniques
Protease Inhibitors / pharmacology*
Skin / enzymology,  pathology
Staining and Labeling
Time Factors
Tissue Distribution
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/CD68 antigen, human; 0/Collagen Type VII; 0/Microfilament Proteins; 0/Protease Inhibitors; 0/fibrillin; 154-23-4/Catechin; 989-51-5/epigallocatechin gallate; EC Metalloproteinase 7

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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