Document Detail

Epigallocatechin gallate up-regulation of miR-16 and induction of apoptosis in human cancer cells.
MedLine Citation:
PMID:  19269153     Owner:  NLM     Status:  MEDLINE    
Epigallocatechin gallate (EGCG) is a major type of green tea polyphenols and is known to have cancer prevention effect. MicroRNAs (miRNAs) are 19 to 25 nucleotides and are believed to be important in gene regulation. In the present study, the influence of EGCG on the expressions of miRNAs in human cancer cells was investigated as this has not yet been reported. By miRNA microarray analysis, EGCG treatment was found to modify the expressions of some of the miRNAs in human hepatocellular carcinoma HepG2 cells, 13 were up-regulated and 48 were down-regulated. miR-16 is one of the miRNAs up-regulated by EGCG and one of its target genes is confirmed to be the anti-apoptotic protein Bcl-2. EGCG treatment induced apoptosis and down-regulated Bcl-2 in HepG2 cells. Transfection with anti-miR-16 inhibitor suppressed miR-16 expression and counteracted the EGCG effects on Bcl-2 down-regulation and also induction of apoptosis in cells. Results from the present study confirm the role of miR-16 in mediating the apoptotic effect of EGCG and also support the importance of miRNAs in the regulation of the biological activity of EGCG.
Wing Pui Tsang; Tim Tak Kwok
Related Documents :
20338643 - Chosen factors of t and b cell apoptosis in hypertrophic adenoid in children with otiti...
8822793 - Bcl-2: antidote for cell death.
22764833 - Thymic stromal lymphopoietin stimulates the formation of eosinophil extracellular traps.
Publication Detail:
Type:  Journal Article     Date:  2009-03-09
Journal Detail:
Title:  The Journal of nutritional biochemistry     Volume:  21     ISSN:  1873-4847     ISO Abbreviation:  J. Nutr. Biochem.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-04-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010081     Medline TA:  J Nutr Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  140-6     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Anticarcinogenic Agents / pharmacology*
Apoptosis / drug effects*
Carcinoma, Hepatocellular / metabolism*
Catechin / analogs & derivatives*,  pharmacology
DNA Fragmentation / drug effects
Gene Expression Profiling
Gene Expression Regulation, Neoplastic / drug effects
Genes, Reporter
Genes, bcl-2 / drug effects
Hep G2 Cells
Liver Neoplasms / metabolism*
MicroRNAs / antagonists & inhibitors,  genetics,  metabolism*
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Proto-Oncogene Proteins c-bcl-2 / genetics,  metabolism
Tea / chemistry
Up-Regulation / drug effects*
Reg. No./Substance:
0/Anticarcinogenic Agents; 0/MIRN16 microRNA, human; 0/MicroRNAs; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tea; 154-23-4/Catechin; 989-51-5/epigallocatechin gallate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Yogurt protects against growth retardation in weanling rats fed diets high in phytic acid.
Next Document:  Zinc retention differs between primary and transformed cells in response to zinc deprivation.