Document Detail

Epigallocatechin activates haem oxygenase-1 expression via protein kinase Cdelta and Nrf2.
MedLine Citation:
PMID:  18586007     Owner:  NLM     Status:  MEDLINE    
The Nrf2/anti-oxidant response element (ARE) pathway plays an important role in regulating cellular anti-oxidants, including haem oxygenase-1 (HO-1). Various kinases have been implicated in the pathways leading to Nrf2 activation. Here, we investigated the effect of epigallocatechin (EGC) on ARE-mediated gene expression in human monocytic cells. EGC time and dose dependently increased HO-1 mRNA and protein expression but had minimal effect on expression of other ARE-regulated genes, including NAD(P)H:quinone oxidoreductase 1, glutathione cysteine ligase and ferritin. siRNA knock down of Nrf2 significantly inhibited EGC-induced HO-1 expression. Furthermore, inhibition of PKC by Ro-31-8220 dose dependently decreased EGC-induced HO-1 mRNA expression, whereas MAP kinase and phosphatidylinositol-3-kinase pathway inhibitors had no significant effect. EGC stimulated phosphorylation of PKCalphabeta and delta in THP-1 cells. PKCdelta inhibition significantly decreased EGC-induced HO-1 mRNA expression, whereas PKCalpha- and beta-specific inhibitors had no significant effect. These results demonstrate for the first time that EGC-induced HO-1 expression occurs via PKCdelta and Nrf2.
Richard M Ogborne; Stuart A Rushworth; Maria A O'Connell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-27
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  373     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-07-28     Completed Date:  2008-08-25     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  584-8     Citation Subset:  IM    
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MeSH Terms
Catechin / analogs & derivatives*,  pharmacology
Cell Line, Tumor
Gene Expression / drug effects
Gene Expression Regulation
Heme Oxygenase-1 / genetics*
Monocytes / drug effects*,  metabolism
NF-E2-Related Factor 2 / genetics,  metabolism*
Oxidative Stress
Protein Kinase C-delta / antagonists & inhibitors,  metabolism*
RNA, Messenger / metabolism
RNA, Small Interfering / genetics
Grant Support
MC_U105960399//Medical Research Council; //Medical Research Council
Reg. No./Substance:
0/NF-E2-Related Factor 2; 0/NFE2L2 protein, human; 0/RNA, Messenger; 0/RNA, Small Interfering; 1617-55-6/gallocatechol; 8R1V1STN48/Catechin; EC Oxygenase-1; EC Kinase C-delta

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