| Epigallocatechin-3 gallate induces growth inhibition and apoptosis in human breast cancer cells through survivin suppression. | |
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MedLine Citation:
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PMID: 17786300 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent investigations have demonstrated that polyphenolic catechins inhibit cancer cell proliferation and tumor growth. However, how the major active component of tea catechins, epigallocatechin-3 gallate (EGCG), mediates anticancerous effects has not been extensively examined. We have investigated the cell growth inhibitory effects of EGCG on cell growth of the human breast cancer cell line MCF-7, and the mechanism of its action with emphasis on the regulation of tumor cell survival. A significant EGCG dose-dependent growth inhibition was observed coordinated with EGCG-induced apoptosis. Analysis of survivin expression after addition of EGCG showed that both survivin mRNA and protein were decreased. The survivin-promoter luciferase activity in EGCG-treated cells was significantly inhibited by 91+/-2.0% (P<0.001), compared with the control. Interestingly, EGCG strongly inhibited the basal activation of phospho-AKT and AKT kinase activity as early as 30 min after treatment. Furthermore, inhibition of AKT kinase activity by EGCG preceded the suppression of survivin (1 h post treatment), followed by increased caspase-9 activity (6 h post treatment). A dominant negative AKT or the phosphatidylinositol 3-kinase inhibitor, LY294002, also strongly inhibited survivin promoter activity, providing further evidence to support the hypothesis that the inhibitory effect of EGCG on survivin is mediated via the AKT pathway. Therefore, EGCG is a potent proapoptotic agent in MCF-7 breast cancer cells that targets survivin expression via suppression of the AKT pathway. |
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Authors:
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Yan Tang; Daniel Y Zhao; Steven Elliott; Weiqiang Zhao; Tyler J Curiel; Barbara S Beckman; Matthew E Burow |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of oncology Volume: 31 ISSN: 1019-6439 ISO Abbreviation: Int. J. Oncol. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-09-05 Completed Date: 2007-11-08 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: Greece |
Other Details:
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Languages: eng Pagination: 705-11 Citation Subset: IM |
Affiliation:
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Department of Medicine, Section of Hematology and Medical Oncology, Center for Bioenvironmental Research, Tulane Cancer Center, Tulane University Health Sciences Center, New Orleans, LA 70112, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase
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metabolism Anticarcinogenic Agents / pharmacology* Apoptosis / drug effects* Blotting, Western Breast Neoplasms / drug therapy, metabolism, pathology* Catechin / analogs & derivatives*, pharmacology Cell Line, Tumor Colony-Forming Units Assay Gene Expression Regulation, Neoplastic* Humans Luciferases / metabolism Microtubule-Associated Proteins / antagonists & inhibitors*, metabolism Neoplasm Proteins / antagonists & inhibitors*, metabolism Promoter Regions, Genetic Protease Inhibitors / pharmacology Proto-Oncogene Proteins c-akt / metabolism Signal Transduction |
| Chemical | |
Reg. No./Substance:
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0/Anticarcinogenic Agents; 0/BIRC5 protein, human; 0/Microtubule-Associated Proteins; 0/Neoplasm Proteins; 0/Protease Inhibitors; 154-23-4/Catechin; 989-51-5/epigallocatechin gallate; EC 1.13.12.-/Luciferases; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.11.1/Proto-Oncogene Proteins c-akt |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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