Document Detail

(-)-Epigallocatechin 3-O-gallate (EGCG)-induced apoptosis in normal rat kidney interstitial fibroblast (NRK-49F) cells.
MedLine Citation:
PMID:  18670168     Owner:  NLM     Status:  MEDLINE    
(-)-Epigallocatechin 3-O-gallate (EGCG), a major catechin in green tea, suppresses renal failure in animals, and inhibits the growth of mesangial cells and opossum kidney proximal tubular cells. In addition, gallic acid, a structural constituent of this catechin, induces apoptosis in tumor cell lines. However, the effects of catechins on renal fibroblastic cells have not been investigated. In this experiment, the growth of normal rat kidney interstitial fibroblast (NRK-49F) cells was significantly inhibited by EGCG at concentrations higher than 6.25 microM, and almost completely inhibited at concentrations over 200 microM. The numbers of in situ end-labeled (ISEL) cells in cultures treated with EGCG at 6.25 to 200 microM increased dose-dependently. Furthermore, exposure to 6.25 to 50 microM EGCG for 24 hr led to a significant increase in caspase-3 activity compared to the control. These results suggest that EGCG induces apoptosis in NRK-49F cells.
Yohei Miyamoto; Mariko Sano; John L Haylor; A Meguid El Nahas
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Publication Detail:
Type:  Letter    
Journal Detail:
Title:  The Journal of toxicological sciences     Volume:  33     ISSN:  1880-3989     ISO Abbreviation:  J Toxicol Sci     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-01     Completed Date:  2008-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7805798     Medline TA:  J Toxicol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  367-70     Citation Subset:  IM    
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MeSH Terms
Apoptosis / drug effects*
Caspase 3 / metabolism
Catechin / analogs & derivatives*,  pharmacology
Cell Proliferation / drug effects
Cells, Cultured
Fibroblasts / drug effects
Kidney / cytology,  drug effects*
Reg. No./Substance:
154-23-4/Catechin; 989-51-5/epigallocatechin gallate; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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