| (-)-Epigallocatechin 3-O-gallate (EGCG)-induced apoptosis in normal rat kidney interstitial fibroblast (NRK-49F) cells. | |
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MedLine Citation:
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PMID: 18670168 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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(-)-Epigallocatechin 3-O-gallate (EGCG), a major catechin in green tea, suppresses renal failure in animals, and inhibits the growth of mesangial cells and opossum kidney proximal tubular cells. In addition, gallic acid, a structural constituent of this catechin, induces apoptosis in tumor cell lines. However, the effects of catechins on renal fibroblastic cells have not been investigated. In this experiment, the growth of normal rat kidney interstitial fibroblast (NRK-49F) cells was significantly inhibited by EGCG at concentrations higher than 6.25 microM, and almost completely inhibited at concentrations over 200 microM. The numbers of in situ end-labeled (ISEL) cells in cultures treated with EGCG at 6.25 to 200 microM increased dose-dependently. Furthermore, exposure to 6.25 to 50 microM EGCG for 24 hr led to a significant increase in caspase-3 activity compared to the control. These results suggest that EGCG induces apoptosis in NRK-49F cells. |
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Authors:
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Yohei Miyamoto; Mariko Sano; John L Haylor; A Meguid El Nahas |
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Publication Detail:
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Type: Letter |
Journal Detail:
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Title: The Journal of toxicological sciences Volume: 33 ISSN: 1880-3989 ISO Abbreviation: J Toxicol Sci Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-01 Completed Date: 2008-10-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7805798 Medline TA: J Toxicol Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 367-70 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Caspase 3 / metabolism Catechin / analogs & derivatives*, pharmacology Cell Proliferation / drug effects Cells, Cultured Fibroblasts / drug effects Kidney / cytology, drug effects* Rats |
| Chemical | |
Reg. No./Substance:
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154-23-4/Catechin; 989-51-5/epigallocatechin gallate; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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