Document Detail

Epidural anaesthesia restores pancreatic microcirculation and decreases the severity of acute pancreatitis.
MedLine Citation:
PMID:  16521220     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate the effect of epidural anaesthesia (EA) on pancreatic microcirculation during acute pancreatitis (AP).
METHODS: AP was induced by injection of sodium taurocholate into the pancreatic duct of Sprague-Dawley rats. To realize EA, a catheter was introduced into the epidural space between T7 and T9 and bupivacaine was injected. Microcirculatory flow was measured by laser Doppler flowmetry. Arterial blood gas analyses were performed. At the end of the experiment (<or=5 h), pancreas was removed for histology. The animals were divided into three groups: Group 1 (n=9), AP without EA; Group 2 (n=4), EA without AP; and Group 3 (n=6), AP treated by EA.
RESULTS: In Group 1, pancreatic microcirculatory flow prior to AP was 141+/- 39 perfusion units (PU). After AP, microcirculatory flow obviously decreased to 9+/- 6 PU (P<0.05). Metabolic acidosis developed with base excess (BE) of -14+/-3 mmol/L. Histology revealed extensive edema and tissue necrosis. In Group 2, EA did not significantly modify microcirculatory flow. BE remained unchanged and histological analysis showed normal pancreatic tissue. In Group 3, AP initially caused a significant decrease in microcirculatory flow from 155+/-25 to 11+/-7 PU (P<0.05). After initiation of EA, microcirculatory flow obviously increased again to 81+/-31 PU (P<0.05). BE was -6+/-4 mmol/L, which was significantly different compared to Group 1 (P<0.05). Furthermore, histology revealed less extensive edema and necrosis in pancreatic tissue in Group 3 than that in Group 1.
CONCLUSION: AP caused dramatic microcirculatory changes within the pancreas, with development of metabolic acidosis and tissue necrosis. EA allowed partial restoration of microcirculatory flow and prevented development of tissue necrosis and systemic complications. Therefore, EA should be considered as therapeutic option to prevent evolution from edematous to necrotic AP.
Alp Demirag; Catherine M Pastor; Philippe Morel; Copin Jean-Christophe; Andreas W Sielenkämper; Nilgun Güvener; Gang Mai; Thierry Berney; Jean-Louis Frossard; Leo H Bühler
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  12     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-03-07     Completed Date:  2006-05-02     Revised Date:  2014-09-09    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  915-20     Citation Subset:  IM    
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MeSH Terms
Acute Disease
Anesthesia, Epidural* / methods
Disease Models, Animal
Microcirculation / drug effects*
Motor Activity
Pancreas / blood supply*
Pancreatitis / drug therapy*,  physiopathology
Rats, Wistar
Reg. No./Substance:

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