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Epidermal growth factor and thyrotropin-releasing hormone act similarly on a clonal pituitary cell strain. Modulation of hormone production and inhbition of cell proliferation.
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MedLine Citation:
PMID:  6771298     Owner:  NLM     Status:  MEDLINE    
GH(4)C(1) cells are a clonal strain of rat pituitary cells that synthesize and secrete prolactin and growth hormone. Chronic treatment (longer than 24 h) of GH(4)C(1) cells with epidermal growth factor (EGF) (10(-8) M) decreased by 30-40 percent both the rate of cell proliferation and the plateau density reached by cultures. Inhibition of cell proliferation was accompanied by a change in cellular morphology from a spherical appearance to an elongated flattened shape and by a 40-60 percent increase in cell volume. These actions of EGF were qualitatively similar to those of the hypothalamic tripeptide thyrotropin-releasing hormone (TRH) (10(-7) M) which decreased the rate of cell proliferation by 10-20 percent and caused a 15 percent increase in cell volume. The presence of supramaximal concentrations of both EGF (10(-8)M) and TRH (10(-7)M) resulted in greater effects on cell volume and cell multiplication than either peptide alone. EGF also altered hormone production by GH(4)C(1) cells in the same manner as TRH. Treatment of cultures with 10(-8) M EGF for 2-6 d increased prolactin synthesis five- to ninefold compared to a two- to threefold stimulation by 10(-7) M TRH. Growth hormone production by the same cultures was inhibited 40 percent by EGF and 15 percent by TRH. The half- maximal effect of EGF to increase prolactin synthesis, decrease growth hormone production, and inhibit cell proliferation occurred at a concentration of 5 x 10 (-11) M. Insulin and multiplication stimulating activity, two other growth factors tested, did not alter cell proliferation, cell morphology, or hormone production by GH(4)C(1) cells, indicating the specificity of the EGF effect. Fibroblast growth factor, however, had effects similar to those of EGF and TRH. Of five pituitary cell strains tested, all but one responded to chronic EGF treatment with specifically altered hormone production. Acute chronic EGF treatment with specifically altered hormone production. Acute treatment (30 min) of GH(4)C(1) cells with 10(-8) M EGF caused a 30 percent enhancement of prolactin release compared to a greater than twofold increase caused by 10(-7) M TRH. Therefore, although EGF and TRH have qualitatively similar effects on GH(4)C(1) cells, their powers to affect hormone release acutely or hormone synthesis and cell proliferation chronically are distinct.
A Schonbrunn; M Krasnoff; J M Westendorf; A H Tashjian
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  85     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1980 Jun 
Date Detail:
Created Date:  1980-09-26     Completed Date:  1980-09-26     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  786-97     Citation Subset:  IM    
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MeSH Terms
Cell Division / drug effects
Cell Line
DNA / biosynthesis
Epidermal Growth Factor / pharmacology*
Growth Hormone / biosynthesis*
Growth Substances / pharmacology
Peptides / pharmacology*
Pituitary Gland / cytology*,  drug effects,  metabolism*
Prolactin / biosynthesis*,  secretion
Thyrotropin-Releasing Hormone / pharmacology*
Reg. No./Substance:
0/Growth Substances; 0/Peptides; 24305-27-9/Thyrotropin-Releasing Hormone; 62229-50-9/Epidermal Growth Factor; 9002-62-4/Prolactin; 9002-72-6/Growth Hormone; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 6 Year: 1980
Volume: 85 Issue: 3
First Page: 786 Last Page: 797
ID: 2111439
Publisher Id: 853786
PubMed Id: 6771298

Epidermal growth factor and thyrotropin-releasing hormone act similarly on a clonal pituitary cell strain. Modulation of hormone production and inhibition of cell proliferation
A Schonbrunn
Westendorf J Krasnoff M
A Tashjian

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