Document Detail

Epidermal growth factor stimulates corneal epithelial cell attachment to fibronectin through a fibronectin receptor system.
MedLine Citation:
PMID:  1386066     Owner:  NLM     Status:  MEDLINE    
Epidermal growth factor (EGF) stimulates corneal epithelial migration in vivo and in vitro. Antibody against fibronectin inhibits this effect in vitro, suggesting that a fibronectin-dependent mechanism in involved. To elucidate the action of EGF, we placed rabbit corneal epithelial cells, preincubated in the absence or presence of EGF (10 ng/ml), into wells coated with fibronectin. After 45 minutes of incubation, the numbers of cells attached to the wells were counted. Preincubation with EGF for 6 hr was not effective, but preincubation for 9 hr significantly increased the numbers of cells attached to the wells. These numbers were not increased further by additional preincubation. When concentrations of EGF were reduced, numbers of attached cells decreased proportionally, but remained significantly higher than the numbers obtained with cells not exposed to EGF. The EGF-stimulated attachment to the fibronectin matrix was inhibited in a dose-dependent fashion by antifibronectin IgG and by GRGDSP, a synthetic peptide that mimics the amino acid sequence of the cell-binding domain of fibronectin. The authors conclude that a fibronectin/fibronectin receptor system mediates EGF-induced stimulation of cellular attachment. These findings suggest that EGF may increase the expression of fibronectin receptors.
T Nishida; M Nakamura; J Murakami; H Mishima; T Otori
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  33     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-08-21     Completed Date:  1992-08-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2464-9     Citation Subset:  IM    
Department of Ophthalmology, Kinki University School of Medicine, Osaka, Japan.
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MeSH Terms
Amino Acid Sequence
Cell Adhesion / drug effects
Cell Count
Cells, Cultured
Cornea / cytology,  drug effects,  metabolism*
Epidermal Growth Factor / pharmacology*
Epithelial Cells
Epithelium / drug effects,  metabolism
Fibronectins / metabolism*,  pharmacology
Immunoglobulin G
Molecular Sequence Data
Oligopeptides / pharmacology
Receptors, Antigen / metabolism
Receptors, Fibronectin
Receptors, Immunologic / metabolism*
Wound Healing / physiology
Reg. No./Substance:
0/Fibronectins; 0/Immunoglobulin G; 0/Oligopeptides; 0/Receptors, Antigen; 0/Receptors, Fibronectin; 0/Receptors, Immunologic; 62229-50-9/Epidermal Growth Factor

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