| Epidermal growth factor receptors in the human glioblastoma cell line SF268 differ from those in epidermoid carcinoma cell line A431. | |
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MedLine Citation:
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PMID: 2998356 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two established human tumor cell lines, epidermoid carcinoma line A431 and glioblastoma line SF268, were studied to compare the interaction of each with epidermal growth factor (EGF). SF268 cells bound [125I] EGF with 35-40 fold higher affinity than did the A431 cells. The EGF binding sites of both lines were photoaffinity labeled using 2,4-NAPS-[125I] EGF, a photoreactive derivative of EGF. Extracts of photolysed cells analyzed by SDS-PAGE showed a difference between the two cell lines in the high molecular weight component corresponding to the EGF receptor. EGF in a dose range from 0.3-200 nM had no effect on thymidine incorporation by SF268 cells, whereas thymidine incorporation by A431 cells was markedly inhibited by EGF. |
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Authors:
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M Westphal; G R Harsh; M L Rosenblum; R G Hammonds |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 132 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1985 Oct |
Date Detail:
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Created Date: 1985-12-13 Completed Date: 1985-12-13 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 284-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Affinity Labels
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metabolism Binding Sites Carcinoma, Squamous Cell / analysis* Cell Line Chromatography, Gel Chromatography, High Pressure Liquid DNA Replication / drug effects Electrophoresis, Polyacrylamide Gel Epidermal Growth Factor / metabolism Glioma / analysis* Humans Photochemistry Receptor, Epidermal Growth Factor Receptors, Cell Surface / analysis* |
| Grant Support | |
ID/Acronym/Agency:
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CA 09291-07/CA/NCI NIH HHS; CA 13525/CA/NCI NIH HHS; CA 31882/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Affinity Labels; 0/Receptors, Cell Surface; 62229-50-9/Epidermal Growth Factor; EC 2.7.10.1/Receptor, Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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