Document Detail


Epidermal growth factor receptors in the human glioblastoma cell line SF268 differ from those in epidermoid carcinoma cell line A431.
MedLine Citation:
PMID:  2998356     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two established human tumor cell lines, epidermoid carcinoma line A431 and glioblastoma line SF268, were studied to compare the interaction of each with epidermal growth factor (EGF). SF268 cells bound [125I] EGF with 35-40 fold higher affinity than did the A431 cells. The EGF binding sites of both lines were photoaffinity labeled using 2,4-NAPS-[125I] EGF, a photoreactive derivative of EGF. Extracts of photolysed cells analyzed by SDS-PAGE showed a difference between the two cell lines in the high molecular weight component corresponding to the EGF receptor. EGF in a dose range from 0.3-200 nM had no effect on thymidine incorporation by SF268 cells, whereas thymidine incorporation by A431 cells was markedly inhibited by EGF.
Authors:
M Westphal; G R Harsh; M L Rosenblum; R G Hammonds
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  132     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1985-12-13     Completed Date:  1985-12-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  284-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Affinity Labels / metabolism
Binding Sites
Carcinoma, Squamous Cell / analysis*
Cell Line
Chromatography, Gel
Chromatography, High Pressure Liquid
DNA Replication / drug effects
Electrophoresis, Polyacrylamide Gel
Epidermal Growth Factor / metabolism
Glioma / analysis*
Humans
Photochemistry
Receptor, Epidermal Growth Factor
Receptors, Cell Surface / analysis*
Grant Support
ID/Acronym/Agency:
CA 09291-07/CA/NCI NIH HHS; CA 13525/CA/NCI NIH HHS; CA 31882/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Affinity Labels; 0/Receptors, Cell Surface; 62229-50-9/Epidermal Growth Factor; EC 2.7.10.1/Receptor, Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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