Document Detail

Epidermal growth factor receptors in the human glioblastoma cell line SF268 differ from those in epidermoid carcinoma cell line A431.
MedLine Citation:
PMID:  2998356     Owner:  NLM     Status:  MEDLINE    
Two established human tumor cell lines, epidermoid carcinoma line A431 and glioblastoma line SF268, were studied to compare the interaction of each with epidermal growth factor (EGF). SF268 cells bound [125I] EGF with 35-40 fold higher affinity than did the A431 cells. The EGF binding sites of both lines were photoaffinity labeled using 2,4-NAPS-[125I] EGF, a photoreactive derivative of EGF. Extracts of photolysed cells analyzed by SDS-PAGE showed a difference between the two cell lines in the high molecular weight component corresponding to the EGF receptor. EGF in a dose range from 0.3-200 nM had no effect on thymidine incorporation by SF268 cells, whereas thymidine incorporation by A431 cells was markedly inhibited by EGF.
M Westphal; G R Harsh; M L Rosenblum; R G Hammonds
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  132     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1985-12-13     Completed Date:  1985-12-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  284-9     Citation Subset:  IM    
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MeSH Terms
Affinity Labels / metabolism
Binding Sites
Carcinoma, Squamous Cell / analysis*
Cell Line
Chromatography, Gel
Chromatography, High Pressure Liquid
DNA Replication / drug effects
Electrophoresis, Polyacrylamide Gel
Epidermal Growth Factor / metabolism
Glioma / analysis*
Receptor, Epidermal Growth Factor
Receptors, Cell Surface / analysis*
Grant Support
Reg. No./Substance:
0/Affinity Labels; 0/Receptors, Cell Surface; 62229-50-9/Epidermal Growth Factor; EC, Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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